FastQCFastQC Report
Thu 26 May 2016
SRR1565897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565897_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15705233
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT583860.3717614377322514No Hit
GTACATGGGAAGCAGTGGTATCAAC575950.36672489991074947No Hit
GGTATCAACGCAGAGTACTTTTTTT417360.2657458186070847No Hit
TATCAACGCAGAGTACTTTTTTTTT408550.26013622338490616No Hit
CCCATGTACTCTGCGTTGATACCAC378090.24074141402423No Hit
GAGTACATGGGAAGCAGTGGTATCA240560.15317187589639708No Hit
ACGCAGAGTACTTTTTTTTTTTTTT185760.11827904749964549No Hit
GTACTTTTTTTTTTTTTTTTTTTTT173330.11036448806585678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG15600.08.6521194
GTATAAT21350.08.5567881
AAGGCGT10550.08.5541456
TAAGACT18750.08.4152214
GTCCTAA15900.08.3779621
GTATTAG11250.08.2885971
TCTATAC14250.08.2710643
TATACTG21750.08.2596695
GTCCTAC21150.08.2328111
ACGATAC3350.08.2282553
GTATTAA20550.08.1953751
TGCGTTG98350.08.16053512
GTATAGA17700.08.1172911
TTATACT18750.08.1110564
TAGTACG1659.689475E-68.0649714
CGATACT3550.08.03251654
CTGCGTT101150.07.963366511
TAATACT18650.07.95068364
ATAAGAC11850.07.9409313
GTATAAG18350.07.9334631
Produced by FastQC (version 0.11.5)