FastQCFastQC Report
Thu 26 May 2016
SRR1513230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5985325
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT220660.36866836805018943No Hit
GGTATCAACGCAGAGTACTTTTTTT155130.2591839206726452No Hit
TATCAACGCAGAGTACTTTTTTTTT135540.22645386841984352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.015.3295661
GTATCAA83600.010.8858971
GTCAACG2301.8189894E-1210.5642372
CGTCTAG954.5903277E-410.4631431
GTCCTAG7600.09.7365361
GTGGTAT13000.09.6001581
GTCCTAA10750.09.4519771
GCACCGT2401.8189894E-129.3461676
TAGCACG1003.2486624E-49.3461674
GACCGTC1901.4169927E-99.3459337
TGGTATC12350.09.3005722
TAGGACC8600.09.2374914
CGTTAGG1203.788934E-49.20369151
TTAGGAC12150.09.0769363
AGGACGT14750.08.9343015
ACCGTCC2950.08.8707158
GTATAGA8100.08.8628141
GGACGTG14900.08.7816336
GTAGGAC14800.08.7778193
GTCCTAC13750.08.755221
Produced by FastQC (version 0.11.5)