FastQCFastQC Report
Thu 26 May 2016
SRR1513230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5985325
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT294380.491836282908614No Hit
GGTATCAACGCAGAGTACTTTTTTT205770.3437908551331799No Hit
TATCAACGCAGAGTACTTTTTTTTT188600.3151040252617861No Hit
ACGCAGAGTACTTTTTTTTTTTTTT86890.1451717325291442No Hit
GTACTTTTTTTTTTTTTTTTTTTTT74470.12442097964605098No Hit
GAGTACTTTTTTTTTTTTTTTTTTT60500.1010805595351965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49400.014.8492281
TAGATCG700.001491471510.8580225
TACGACT1451.4446414E-810.4836074
GTGGTAT12300.010.3921191
CGTGCGC2250.010.12975110
GTATCAA102900.09.9840051
AAGGCGT4500.09.9187156
TGGTATC11850.09.8615572
GTAGGAC17500.09.55505853
TAAGACG1002.755547E-49.5007694
GTCCTAA10900.09.3640071
GTCCTAC14200.09.2031541
GTATAAA9650.09.193061
TAGGACC7900.09.1399814
GTATTAA7900.08.8145521
ATAACGC1201.7638545E-48.7090383
ACACCGT3000.08.5469776
TAGGACT7050.08.4900484
TGTAGGA21350.08.4550162
TCGAACT4950.08.44251719
Produced by FastQC (version 0.11.5)