FastQCFastQC Report
Thu 26 May 2016
SRR1513229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5101155
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT222940.43703827858592814No Hit
GGTATCAACGCAGAGTACTTTTTTT142740.27981898217168466No Hit
TATCAACGCAGAGTACTTTTTTTTT141820.2780154690457357No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59800.11722835318668026No Hit
GTACTTTTTTTTTTTTTTTTTTTTT57780.11326846567100982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34350.015.2351211
CGTCTAT1501.7644197E-1012.5127271
GTATCAA74100.011.7707231
CGTTATG1006.033924E-511.0406422
GCGTATT902.7934255E-411.0406421
TAGGACG15850.010.08365254
GGACGTG16100.010.043186
CGTTAGG1007.382477E-49.9365781
GTCTTAC4000.09.9365781
AGGACGT16000.09.9307025
GTCTAGA5900.09.9178641
TAGACGG857.663191E-49.896345
ACCGATC857.66388E-49.8962438
GTCCTAA10150.09.6809571
ACCGTCC1452.0534571E-79.6687438
GTAGGAC16650.09.6552893
GTATAAT7350.09.613621
GCGTTGA1856.141818E-89.5486631
GTCAACG2209.858923E-109.53512
TGTAGGA16950.09.50993252
Produced by FastQC (version 0.11.5)