FastQCFastQC Report
Thu 26 May 2016
SRR1513229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5101155
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT312250.6121162756277745No Hit
TATCAACGCAGAGTACTTTTTTTTT202980.3979098851142535No Hit
GGTATCAACGCAGAGTACTTTTTTT197720.38759849485067593No Hit
ACGCAGAGTACTTTTTTTTTTTTTT90460.17733238844928256No Hit
GTACTTTTTTTTTTTTTTTTTTTTT78460.1538083041977748No Hit
GAGTACTTTTTTTTTTTTTTTTTTT62370.12226642789721152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42650.014.3376591
CGACCGT500.001503863813.29589114
GTGGTAT11800.010.5099481
GTATCAA92950.010.4173161
GTCCTAC15900.010.1397871
TGGTATC10350.09.9138682
GTCTAGA4850.09.8348441
TAGGACG15300.09.5628874
CGTCGTA1002.767813E-49.49650710
AGGACGT15900.09.0825165
GTAGGAC16000.09.025753
GTCTTAT5400.09.009811
CGTGCGC2750.08.97851510
TAGGACC7100.08.9655334
TCCTACA20250.08.9612392
GGACGTG15400.08.9416036
GCGCCAC2451.6370905E-118.91561113
GTTCTAA5400.08.8331481
GTGTTAG5750.08.7932061
GTCCTAA11000.08.7592711
Produced by FastQC (version 0.11.5)