FastQCFastQC Report
Thu 26 May 2016
SRR1513228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5086185
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT137270.26988794155147716No Hit
GGTATCAACGCAGAGTACTTTTTTT97460.19161709611427818No Hit
TATCAACGCAGAGTACTTTTTTTTT84480.1660969862480425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.014.2432811
GTCAACG1750.013.290972
GTATCAA54450.011.2283681
GTGGTAT9000.011.1988731
CGTGCGG700.001714451910.67799110
CGCATAG1203.299788E-510.1528231
GTCTAGG4900.09.9456231
TGGTATC9200.09.8719162
TACCGTG1503.3765718E-79.3432437
TAGGACG5600.09.176674
CGTGCGC2055.802576E-109.11535910
GTCTAAG4650.09.0511981
TAAGACT5800.09.0213284
CGTCTAG1255.5376836E-48.8606461
GTATTAT6800.08.7954941
TATATCG850.0085243848.79395
TCGATAC850.0085243848.79393
GTAGGAC8400.08.7873563
TAAAGGT8750.08.6494284
CGATAAA1555.6869125E-58.574821
Produced by FastQC (version 0.11.5)