FastQCFastQC Report
Thu 26 May 2016
SRR1513228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5086185
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT196200.3857508132323146No Hit
GGTATCAACGCAGAGTACTTTTTTT138680.2726601568759296No Hit
TATCAACGCAGAGTACTTTTTTTTT127360.25040378987394285No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58500.11501744431238739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35800.013.6654471
GTGGTAT11500.010.0340681
GTATCAA71900.09.7885181
AACACCG1401.0589883E-69.5006845
TTAACGT800.0045157499.5006834
TTAACCG950.00182374759.0006484
TTAGGAC7000.08.95778753
GTATAGG4700.08.9277971
GTATTAA6950.08.9190391
GCGTAGG1106.8816455E-48.6695561
CTAATAC3950.08.6588513
ACCGTGC2201.6189006E-98.6334198
TGGTATC11900.08.4627932
TAGCACG1356.7984794E-58.4450524
GTAGGAC7800.08.1608443
GACGTGA4200.08.140087
TAGGACG5500.08.1187664
TAGGACC4800.08.1151684
TAGGACA7500.08.107254
TAATACT8350.07.9646454
Produced by FastQC (version 0.11.5)