##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513228_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5086185 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.71639686720007 31.0 31.0 34.0 27.0 34.0 2 30.892024965666803 31.0 31.0 34.0 27.0 34.0 3 30.98662769836331 31.0 31.0 34.0 27.0 34.0 4 34.61346942747855 37.0 35.0 37.0 32.0 37.0 5 34.26708269557635 37.0 35.0 37.0 30.0 37.0 6 34.244960220676205 37.0 35.0 37.0 30.0 37.0 7 34.094880544062 37.0 35.0 37.0 30.0 37.0 8 33.98774641504389 37.0 35.0 37.0 30.0 37.0 9 35.461186921042 39.0 35.0 39.0 30.0 39.0 10 35.2078713220223 38.0 35.0 39.0 30.0 39.0 11 35.26646867937364 38.0 35.0 39.0 30.0 39.0 12 35.03822904593522 38.0 35.0 39.0 29.0 39.0 13 35.0323411358415 38.0 35.0 39.0 29.0 39.0 14 35.95399026972082 39.0 36.0 41.0 28.0 41.0 15 35.857905483186315 39.0 36.0 40.0 27.0 41.0 16 35.735949636122164 39.0 36.0 40.0 27.0 41.0 17 35.666806063876955 39.0 36.0 40.0 27.0 41.0 18 35.56184507641779 39.0 36.0 40.0 27.0 41.0 19 35.56185844596687 39.0 36.0 40.0 27.0 41.0 20 35.42687613604303 39.0 35.0 40.0 27.0 41.0 21 35.30988137474355 39.0 35.0 40.0 27.0 41.0 22 35.163957465172814 39.0 35.0 40.0 27.0 41.0 23 35.257452098183606 39.0 35.0 40.0 27.0 41.0 24 35.11990283483593 39.0 35.0 40.0 27.0 41.0 25 34.61778602233304 38.0 34.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05381419085343353 1101 2 -0.014448775239852552 1101 3 0.024098267032673704 1101 4 0.05061038180993194 1101 5 0.06763852737891796 1101 6 0.057616424353000184 1101 7 -0.036072177230209945 1101 8 -0.06083394977788714 1101 9 -0.09430878138019949 1101 10 -0.14585990197716114 1101 11 -0.18861347980773502 1101 12 -0.14057465774367017 1101 13 -0.10508220260655321 1101 14 -0.02482244994921956 1101 15 -0.10233918338953174 1101 16 -0.2564861604170332 1101 17 -0.1531231959712187 1101 18 -0.2884907639833685 1101 19 -0.3916993463785161 1101 20 -0.34704602510198157 1101 21 -0.30122578794530597 1101 22 -0.4493809191254883 1101 23 -0.5087656425261429 1101 24 -0.7215803867263375 1101 25 -0.7716534019750156 1102 1 0.32894336356939746 1102 2 0.2769342766668643 1102 3 0.3183442243487207 1102 4 0.3872744512813213 1102 5 0.3471473644954486 1102 6 0.47835443024420954 1102 7 0.4837088278092523 1102 8 0.2809784730529472 1102 9 0.4379334520352316 1102 10 0.6915290763797017 1102 11 0.5144570216368365 1102 12 0.55326541194448 1102 13 0.5662813854759392 1102 14 0.6827362849344496 1102 15 0.8605824949510108 1102 16 0.7884082469405058 1102 17 0.7636816379973794 1102 18 0.8156968132644451 1102 19 0.7705019809163431 1102 20 0.7839080660468767 1102 21 0.5207589896562936 1102 22 0.6952091747577498 1102 23 0.8488504507460561 1102 24 0.8750859880131188 1102 25 1.0574157294434698 1103 1 0.29919421171243954 1103 2 0.25009224525057405 1103 3 0.29915614338281316 1103 4 0.3361338393342308 1103 5 0.3018955001699055 1103 6 0.2272503449669756 1103 7 0.31995662844294515 1103 8 0.2811214972456 1103 9 0.2189405739372816 1103 10 0.4286702551529942 1103 11 0.24515716059629966 1103 12 0.4178928938155053 1103 13 0.41728180017423 1103 14 0.5579452412036261 1103 15 0.34016373512807974 1103 16 0.5335349032081709 1103 17 0.40728802935005604 1103 18 0.465086221344734 1103 19 0.4470959320391259 1103 20 0.31192635365838584 1103 21 0.47760509298971954 1103 22 0.4939655909819507 1103 23 0.419310149347524 1103 24 0.5163891388496538 1103 25 0.42835711851263625 1104 1 0.04975633028736581 1104 2 0.10924877390558407 1104 3 0.15126523098398081 1104 4 0.16337805170525854 1104 5 0.15736969907347031 1104 6 0.2613287031855265 1104 7 0.23425351076905088 1104 8 0.15574081756794556 1104 9 0.14702143389042277 1104 10 0.26868762558159887 1104 11 0.3187088122485804 1104 12 0.4248389605990468 1104 13 0.5105664738578213 1104 14 0.5037162036371541 1104 15 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-0.5824097772781514 2313 13 -0.5939236193387458 2313 14 -1.7012380668823184 2313 15 -1.2468374837040415 2313 16 -1.0098151145857983 2313 17 -0.9521122851246417 2313 18 -0.787306683427019 2313 19 -0.7030997803896142 2313 20 -0.6585969747232454 2313 21 -0.6750158495119152 2313 22 -0.6266608843359407 2313 23 -0.5765745298483935 2313 24 -0.5241165712686637 2313 25 -0.47740982857114744 2314 1 -0.4539967365759452 2314 2 -0.4772857046935144 2314 3 -0.49875502510764136 2314 4 -0.5359077503676914 2314 5 -0.5233942969754537 2314 6 -0.5661531232311461 2314 7 -0.6386611359783672 2314 8 -0.5991513699533897 2314 9 -0.5413078709842409 2314 10 -0.5933114913438473 2314 11 -0.7306386684853692 2314 12 -0.6640465902073274 2314 13 -0.6526338975797259 2314 14 -0.7629313449205668 2314 15 -0.721861701963455 2314 16 -0.678068181334524 2314 17 -0.6343727958081686 2314 18 -0.6582150308837882 2314 19 -0.622649522418925 2314 20 -0.6280405789851571 2314 21 -0.7135713058597801 2314 22 -0.8164331848499771 2314 23 -0.7336242533229793 2314 24 -0.8517716743250858 2314 25 -0.918240451151199 2315 1 -0.4976851090290104 2315 2 -0.5781074405864679 2315 3 -0.6773548226700576 2315 4 -0.6725016390316938 2315 5 -0.7685137172418166 2315 6 -0.8028733640490557 2315 7 -0.9048315319027296 2315 8 -0.9037299845568327 2315 9 -1.0032383589501492 2315 10 -0.9544709649303371 2315 11 -0.9575014705495946 2315 12 -1.1196807255080046 2315 13 -1.1623278178799339 2315 14 -1.2166267877504922 2315 15 -1.190975537431136 2315 16 -1.226502536752676 2315 17 -1.3924253966706317 2315 18 -1.291487434183118 2315 19 -1.2820003318929025 2315 20 -1.1882180385908825 2315 21 -1.2791788098392587 2315 22 -1.2108985644640313 2315 23 -1.1787805430133105 2315 24 -1.1597685904823294 2315 25 -1.2564527035044364 2316 1 -0.862229380504612 2316 2 -0.8617757635931937 2316 3 -0.924867205911653 2316 4 -0.9366365341017726 2316 5 -0.8993615915772253 2316 6 -1.020769628731074 2316 7 -1.1122448213149667 2316 8 -0.9522008509228144 2316 9 -1.1373333035392434 2316 10 -1.141446817751877 2316 11 -1.1460151239745429 2316 12 -1.4151975311230345 2316 13 -1.4705654140645876 2316 14 -1.5558997425073855 2316 15 -1.2620088012216044 2316 16 -1.2306005487007923 2316 17 -1.3078000804720702 2316 18 -1.285637039642637 2316 19 -1.4589590511240544 2316 20 -1.2820098636923305 2316 21 -1.4752408737090548 2316 22 -1.2267605766743799 2316 23 -1.1958732078153886 2316 24 -1.3051641663082947 2316 25 -1.204307695152572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 89810.0 3 3.0 4 1546.0 5 12390.0 6 15535.0 7 13042.0 8 10977.0 9 9982.0 10 9591.0 11 9238.0 12 8916.0 13 8534.0 14 8436.0 15 8489.0 16 8509.0 17 8980.0 18 9556.0 19 10741.0 20 12986.0 21 15934.0 22 20077.0 23 25030.0 24 30314.0 25 35989.0 26 41671.0 27 47074.0 28 55523.0 29 67723.0 30 85931.0 31 112782.0 32 148669.0 33 199720.0 34 275128.0 35 397776.0 36 644762.0 37 1159213.0 38 1465608.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.10156154219887 19.496761601899035 13.322710758446716 24.07896609745538 2 16.981509717007935 21.672451945810074 37.42600790179673 23.920030435385264 3 19.179424263962087 25.56578260523359 30.362560543904713 24.892232586899613 4 14.09626271950391 17.78879454836975 36.965328630397835 31.149614101728506 5 14.015312519976595 37.57228207687731 35.017835102747306 13.394570300398795 6 32.1714605347623 37.204604236770784 17.426617395946078 13.197317832520838 7 27.971514996013713 32.06916382317985 22.19158367224157 17.767737508564867 8 26.3536675352385 34.873942392708635 20.413804059552582 18.35858601250028 9 26.879851991227216 14.957222358211508 19.555305204195285 38.607620446365985 10 15.284560038614403 28.755403116481215 34.026603436563946 21.933433408340434 11 34.93419920824744 22.512256239204824 23.621791185338324 18.93175336720941 12 23.039173761866707 25.071895733246034 30.92885925305509 20.96007125183217 13 28.98195799012423 21.623259869627233 25.722481584920722 23.672300555327812 14 22.218774975743116 20.97373571743851 27.444715439961385 29.362773866856983 15 24.060351717446377 28.863598158541226 23.6510862267102 23.424963897302202 16 23.6228796689064 27.88065687869568 25.69115297841698 22.80531047398094 17 22.75058811270137 27.657389575880547 26.860289195143316 22.73173311627477 18 22.689123546593077 27.325475653744324 27.666693866074706 22.318706933587894 19 23.69971745019792 26.922468184884423 27.584811635759078 21.793002729158587 20 23.931846757441974 26.41730491517709 27.493514294112387 22.15733403326855 21 24.074311099576597 26.207638927801487 27.230094854984632 22.487955117637288 22 23.915704993035057 26.253449294510524 27.277890992954447 22.55295471949998 23 23.651695720859543 26.299416950032295 27.142661936205627 22.906225392902538 24 23.363306682710125 26.486531653882032 27.408578335235546 22.741583328172293 25 23.575890967145302 26.3898843636817 27.31928912579714 22.714935543375862 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1110.0 1 1110.0 2 1509.5 3 1909.0 4 1909.0 5 1909.0 6 4569.0 7 7229.0 8 7229.0 9 7229.0 10 9854.0 11 12479.0 12 12479.0 13 12479.0 14 18380.0 15 24281.0 16 24281.0 17 24281.0 18 42571.0 19 60861.0 20 60861.0 21 60861.0 22 89772.5 23 118684.0 24 118684.0 25 118684.0 26 164743.0 27 210802.0 28 210802.0 29 210802.0 30 263847.5 31 316893.0 32 316893.0 33 316893.0 34 370010.0 35 423127.0 36 423127.0 37 423127.0 38 473826.5 39 524526.0 40 524526.0 41 524526.0 42 566652.5 43 608779.0 44 608779.0 45 608779.0 46 634394.0 47 660009.0 48 660009.0 49 660009.0 50 655674.0 51 651339.0 52 651339.0 53 651339.0 54 599556.0 55 547773.0 56 547773.0 57 547773.0 58 475063.0 59 402353.0 60 402353.0 61 402353.0 62 331597.0 63 260841.0 64 260841.0 65 260841.0 66 202602.0 67 144363.0 68 144363.0 69 144363.0 70 106071.0 71 67779.0 72 67779.0 73 67779.0 74 47487.0 75 27195.0 76 27195.0 77 27195.0 78 18477.5 79 9760.0 80 9760.0 81 9760.0 82 6381.0 83 3002.0 84 3002.0 85 3002.0 86 1944.5 87 887.0 88 887.0 89 887.0 90 525.5 91 164.0 92 164.0 93 164.0 94 100.0 95 36.0 96 36.0 97 36.0 98 20.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4103665124253247 2 0.0 3 0.0 4 0.0 5 0.041484924358826905 6 0.0 7 0.0 8 0.0011403438923279431 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.966110159186109E-4 17 0.0 18 0.005701719461639716 19 3.932220318372218E-5 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.013212260269730654 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5086185.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.25054022999237 #Duplication Level Percentage of deduplicated Percentage of total 1 78.92987460686983 52.291468329906834 2 13.542972839914594 17.94458533928912 3 3.6541505014661766 7.2626833441149525 4 1.4254196468516511 3.7773928663346736 5 0.7092370983799988 2.3493670459413583 6 0.4470246488292272 1.7769374688635358 7 0.28205768745177207 1.3080531918791567 8 0.19311412726861374 1.023513220607133 9 0.1394681570718281 0.831585667580104 >10 0.6395896891387486 7.211799261979225 >50 0.026109877891486423 1.1779031021281903 >100 0.009933529854419035 1.145501045532049 >500 4.489676343774691E-4 0.18943175456545483 >1k 4.7889710183012625E-4 0.6771772480733717 >5k 2.9931068863986083E-5 0.11600254469116063 >10k+ 8.979320659195823E-5 0.9165985685135402 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 19620 0.3857508132323146 No Hit GGTATCAACGCAGAGTACTTTTTTT 13868 0.2726601568759296 No Hit TATCAACGCAGAGTACTTTTTTTTT 12736 0.25040378987394285 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5850 0.11501744431238739 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.966110159186109E-5 0.0 0.0 0.0 6 0.0 1.966110159186109E-5 0.0 0.0 0.0 7 0.0 1.966110159186109E-5 0.0 0.0 0.0 8 0.0 1.966110159186109E-5 0.0 0.0 0.0 9 0.0 1.966110159186109E-5 0.0 1.966110159186109E-5 1.966110159186109E-5 10 0.0 1.966110159186109E-5 0.0 1.966110159186109E-5 1.966110159186109E-5 11 0.0 1.966110159186109E-5 0.0 3.932220318372218E-5 1.966110159186109E-5 12 0.0 1.966110159186109E-5 0.0 3.932220318372218E-5 9.830550795930545E-5 13 0.0 1.966110159186109E-5 0.0 3.932220318372218E-5 1.1796660955116654E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3580 0.0 13.665447 1 GTGGTAT 1150 0.0 10.034068 1 GTATCAA 7190 0.0 9.788518 1 AACACCG 140 1.0589883E-6 9.500684 5 TTAACGT 80 0.004515749 9.500683 4 TTAACCG 95 0.0018237475 9.000648 4 TTAGGAC 700 0.0 8.9577875 3 GTATAGG 470 0.0 8.927797 1 GTATTAA 695 0.0 8.919039 1 GCGTAGG 110 6.8816455E-4 8.669556 1 CTAATAC 395 0.0 8.658851 3 ACCGTGC 220 1.6189006E-9 8.633419 8 TGGTATC 1190 0.0 8.462793 2 TAGCACG 135 6.7984794E-5 8.445052 4 GTAGGAC 780 0.0 8.160844 3 GACGTGA 420 0.0 8.14008 7 TAGGACG 550 0.0 8.118766 4 TAGGACC 480 0.0 8.115168 4 TAGGACA 750 0.0 8.10725 4 TAATACT 835 0.0 7.964645 4 >>END_MODULE