FastQCFastQC Report
Thu 26 May 2016
SRR1513227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7762761
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT134350.17306986521934656No Hit
GGTATCAACGCAGAGTACTTTTTTT85120.10965170768493324No Hit
TATCAACGCAGAGTACTTTTTTTTT83890.1080672198976627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33500.014.4034171
GTATCAA69450.011.5582231
TAGGACC9450.09.7913784
GTCCTAC14100.09.47534751
TAGGACG11700.09.2664324
GTATTAA8950.09.2526711
GTAGGAC15250.09.1318093
GTGGTAT12500.09.0982131
TATACCG2003.5706762E-98.8795
GTATAAT10200.08.8765161
ATAAGAC7200.08.8270763
GTATAAA10800.08.7923221
GTGTAGG10350.08.747871
GTATAGG7500.08.6859961
CGATAAT1401.8393146E-48.675481
TTAGGAC11000.08.6665843
CGCTTAA1308.4395066E-48.4934781
TAATACT10400.08.3577634
GTCTTAG9250.08.3557451
GTCTTAA8300.08.2478831
Produced by FastQC (version 0.11.5)