FastQCFastQC Report
Thu 26 May 2016
SRR1513227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7762761
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT185960.23955394221205573No Hit
TATCAACGCAGAGTACTTTTTTTTT117060.1507968620958445No Hit
GGTATCAACGCAGAGTACTTTTTTT115380.1486326836546945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40000.013.4524231
CCGATCG855.349484E-511.1722579
GTATCAA84500.010.30851
GTTCTAA7300.09.2793361
GTGGTAT14250.09.1724871
TAGGACG10800.09.1493554
TACCGTC1701.6974627E-78.9379797
TTAGGAC11000.08.6375033
GTAGGAC14550.08.6196943
GTCCTAA9650.08.5026161
CTAAGAC7050.08.4904823
GTCTTAT6500.08.3664831
ACCGCGC1252.7565967E-48.357018
TAATACT10950.08.3298664
GTATAAG8300.08.2762921
GTATTAA10500.08.2686291
AGGACCG5450.08.1937415
ATTATAC8850.08.1592683
GGACGTG11900.08.1399446
AGGACGT11950.08.1098575
Produced by FastQC (version 0.11.5)