FastQCFastQC Report
Thu 26 May 2016
SRR1513226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6296530
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT247620.3932642264866522No Hit
GGTATCAACGCAGAGTACTTTTTTT178280.2831400787417832No Hit
TATCAACGCAGAGTACTTTTTTTTT156840.24908957791037284No Hit
ACGCAGAGTACTTTTTTTTTTTTTT68830.1093141778090472No Hit
GTACTTTTTTTTTTTTTTTTTTTTT63760.10126212374117173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43400.015.7475091
GTGGTAT12200.011.0410641
GCGTATA800.00129717911.0410641
GTATCAA85400.010.9634931
GTCAACG2450.010.8157372
GTTCTAA7450.09.9295471
TGGTATC12150.09.9051522
GTCCTAC11900.09.4637691
TAGACCG1305.2220057E-69.3464455
CCGTGCG1608.5450665E-89.3464459
GTCCTAG7350.09.3135511
CTAATAC4750.08.85452653
GCGTAAT1000.0082214398.8328511
GGTCTAA2902.5465852E-118.7567051
GTCTTAG6950.08.7375331
CGTTTAA2156.58074E-88.7301441
GGACCGT2253.619789E-108.723356
TCCTACA13000.08.5780572
GTTACAC5150.08.5297653
TATACTG11250.08.391035
Produced by FastQC (version 0.11.5)