FastQCFastQC Report
Thu 26 May 2016
SRR1513226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6296530
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT332930.5287515504571566No Hit
GGTATCAACGCAGAGTACTTTTTTT233080.3701721424340073No Hit
TATCAACGCAGAGTACTTTTTTTTT210090.33365996826823663No Hit
ACGCAGAGTACTTTTTTTTTTTTTT97220.1544025042364604No Hit
GTACTTTTTTTTTTTTTTTTTTTTT86100.1367419832828558No Hit
GAGTACTTTTTTTTTTTTTTTTTTT69400.11021943832555392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52150.014.5993321
GTGGTAT13400.010.1103911
GTATCAA108550.09.9494951
GTCCTAA9600.09.6401751
TATATCG900.0011137629.5006955
GTCCTAC13450.09.1506481
TAACGCA1252.7548147E-59.12066654
TGGTATC13250.09.1063262
ATAACGC1151.1108356E-49.0876223
GTAGGAC14350.08.8055233
GTATAAG7750.08.7405971
TAAGACG1201.7640412E-48.7089714
GTATAGG6150.08.687551
GGCGTGC4250.08.4909628
AATACCG1252.743589E-48.3606115
GTATTAG4800.08.3481921
TAGGACC6750.08.3043114
TAATACC5150.08.3015784
CAGGACT12700.08.154144
TTAGACG1553.898668E-57.96832474
Produced by FastQC (version 0.11.5)