FastQCFastQC Report
Thu 26 May 2016
SRR1513225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6708423
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT237120.3534660828632899No Hit
GGTATCAACGCAGAGTACTTTTTTT144900.215997112883311No Hit
TATCAACGCAGAGTACTTTTTTTTT144390.21523687459780041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39050.015.51594451
CGATTTA559.800938E-414.0719421
GTATCAA83750.011.8552361
CGGTAAG1152.1042395E-510.5758091
GTGGTAT12700.010.2729931
TGGTATC11300.09.7845362
GTATAGA9550.09.4935621
GTCCTAC17300.09.203121
CGGTAGG1452.6515601E-59.15022851
TAGGACC8650.09.0750424
CCGCGTC1651.3745012E-79.0618119
TAGGACG16100.08.9388234
GTAGGAC17950.08.9026043
CTTACAC7700.08.8596713
GTATTAG6700.08.7462071
CTTATAC6950.08.7400513
GTCCTAA9750.08.7318211
ACCGCTC1952.1871529E-88.6261478
AGGACGT16750.08.5919435
TCTATAC6400.08.4690273
Produced by FastQC (version 0.11.5)