FastQCFastQC Report
Thu 26 May 2016
SRR1513225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6708423
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT341270.5087186660709976No Hit
TATCAACGCAGAGTACTTTTTTTTT214340.319508772777149No Hit
GGTATCAACGCAGAGTACTTTTTTT211790.31570758134959587No Hit
ACGCAGAGTACTTTTTTTTTTTTTT100030.14911105039142583No Hit
GTACTTTTTTTTTTTTTTTTTTTTT80840.12050522156995766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.014.7301471
CGCGCGT551.9678051E-413.81310210
GTATCAA105600.010.2632141
GTGGTAT13500.010.2471531
GTCCTAC15800.09.9027491
TGGTATC12950.09.6108052
GTCCTAA11400.09.2057011
TAGGACC8650.08.8966634
CTTATAC7650.08.8176973
TAGACTG7550.08.8086495
CCGCGCG2054.1782187E-98.8016429
TTATACT9900.08.6370524
ATTATAC8200.08.5738543
GTATAAG7150.08.5397061
GTAGGAC18950.08.4228343
GTATTAA10150.08.36552051
GTATTAG4600.08.2960451
GTATAAA12150.08.1663141
TATACTG11900.08.1435065
CCGTCCG1050.00453084478.1398659
Produced by FastQC (version 0.11.5)