FastQCFastQC Report
Thu 26 May 2016
SRR1513224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6995618
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT160000.22871460391347842No Hit
GGTATCAACGCAGAGTACTTTTTTT112100.1602431693668808No Hit
TATCAACGCAGAGTACTTTTTTTTT96410.13781484352061532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36050.014.3916741
GTATCAA72050.011.2761961
CTAATAC4600.011.1753533
GTGGTAT12350.011.1727941
GCGCTAA850.00215360610.3893841
TGGTATC12350.010.3683542
GTCCTAC8050.09.3246341
GTCTTAG6850.09.1855051
GCGTGCG2359.094947E-129.1477299
GTATTAA9100.08.97654151
GTATTAC5450.08.9119951
TAATACT10400.08.89734
GTCTAGA6950.08.8945081
CGCTTAA1504.0274215E-58.83097651
GTCCTAG8150.08.5329991
GTCTAGG6500.08.4913231
GTCTTAT6150.08.4360951
ATTACAC8650.08.4282013
GTATAAA11050.08.2915281
CGTGCGC3500.08.2784110
Produced by FastQC (version 0.11.5)