FastQCFastQC Report
Thu 26 May 2016
SRR1513224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6995618
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT216560.30956521639689305No Hit
GGTATCAACGCAGAGTACTTTTTTT148590.21240439372189845No Hit
TATCAACGCAGAGTACTTTTTTTTT133630.19101957825598825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40200.013.3869161
TAGACCG1658.0035534E-1110.9406295
GTATCAA84100.010.1770981
GTGGTAT14350.09.8409571
CTAACCT5750.09.7489264
TGGTATC13050.09.464672
GTATTAG6400.08.945381
GTATTAA9200.08.9194521
GTCTTAA7950.08.4015311
GTGTTAG7900.08.2131411
GTCCTAA6800.08.1385411
GTCCTAC7750.08.1258681
TAGGACG4850.08.0318354
TAGGACA10700.07.9915564
TTAGGAC8100.07.97620873
GTCCTAG7200.07.9514491
CTAATAC5550.07.87476353
CTTAGAC7250.07.8629563
CTTATAC8250.07.83118773
TAGAACA9000.07.81199364
Produced by FastQC (version 0.11.5)