FastQCFastQC Report
Thu 26 May 2016
SRR1513223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6529601
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT134940.20665887548105924No Hit
GGTATCAACGCAGAGTACTTTTTTT93110.1425967681639353No Hit
TATCAACGCAGAGTACTTTTTTTTT80400.1231315665382923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30700.015.1942541
GTATCAA61200.010.847321
GTGGTAT12100.010.0250081
GTATTAA8800.09.14781951
GTCAACG2251.4912075E-88.8220072
TGGTATC10800.08.6790582
GTTCGCA1952.1775122E-88.6283839
CTTACAC8850.08.5553933
GTAAGAC4750.08.4623
TCTTATA6150.08.4275262
ATACGCC1000.00336493648.4128043
TAGGCCG1358.2203405E-58.3089415
TAGGACA7250.08.2516384
GTCTTAT6050.08.2022791
CTAGACA7000.08.145734
GTAGGAC5750.08.1283123
TAGACTG8300.08.1087265
CTAGTAG3350.08.0919163
CCGTGCG2201.9570507E-88.0727689
TATACTG9650.08.0398695
Produced by FastQC (version 0.11.5)