FastQCFastQC Report
Thu 26 May 2016
SRR1513223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6529601
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT176710.27062909356942333No Hit
GGTATCAACGCAGAGTACTTTTTTT122470.18756123077045594No Hit
TATCAACGCAGAGTACTTTTTTTTT107070.1639763287220766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38600.013.8930241
GTATCAA77800.09.5912441
GTGGTAT12850.09.5050551
CCTCGCG800.00453298459.496379
AGAACCG3500.08.9574245
TATGCCG1502.7043752E-68.8669455
GTATAGA8400.08.7470031
CGCAACG1000.00291482738.54745317
ATTAGAC3700.08.4732393
GTAGGAC7100.08.4298433
GGACCGT3500.08.4111366
TAGACTG8100.08.3274225
GTATTAT8850.08.3022391
GTATTAA8100.08.2463331
TGGTATC12900.08.1746762
TCTATAC6700.08.0823433
GTCTAGG5550.08.0807691
TAATACG1659.75225E-68.060864
ACCGGTC1304.2061723E-48.0354518
GTCTTAC4900.07.98427631
Produced by FastQC (version 0.11.5)