FastQCFastQC Report
Thu 26 May 2016
SRR1513222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7199233
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT118590.1647258812154017No Hit
GGTATCAACGCAGAGTACTTTTTTT73930.10269149505232014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32000.014.6189831
GTATCAA66300.011.5324341
GTATTAG5550.011.3313881
GTGGTAT11100.010.6356021
CGCTTAT1152.153984E-510.5534911
GTCTTAG7350.09.9073581
TAGGACG12750.09.2371374
GTCCTAA9800.09.1192741
AGGACGT13150.09.0272415
GGTCTAC6900.08.9544761
GTCCTAC15800.08.9382841
TTAGGAC11650.08.9058253
GTTCTAA6500.08.8265561
TGGTATC12000.08.8265562
GTCCTAG8950.08.6293131
TATTAGG5400.08.5813732
TATACTG11850.08.5188795
GTAGGAC15700.08.5136043
CTAGACA6450.08.4051484
TCTTATA7900.08.3796422
Produced by FastQC (version 0.11.5)