FastQCFastQC Report
Thu 26 May 2016
SRR1513222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7199233
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT156430.21728703599397325No Hit
TATCAACGCAGAGTACTTTTTTTTT101620.14115392570291863No Hit
GGTATCAACGCAGAGTACTTTTTTT97760.13579224342370916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40300.013.0934541
GTATCAA84850.010.1885051
TAGGACG13300.09.5008444
GTAGGAC16100.09.2647993
GTGGTAT14450.09.2447321
TAGGACC9150.09.2412584
TAGACTG9050.09.238395
ATAGGAC5900.09.17878153
GTCTAGA7100.09.0043131
TAGGACA11150.08.9469834
TGGTATC13350.08.7535872
TTAGGAC12300.08.7284183
TGGCGAG13550.08.69081618
TATGCCG1752.6264206E-78.6864865
GTCCTAA10600.08.6417071
GTGTTAG7700.08.550521
TATACTG11500.08.5094525
TGTAGGA20100.08.5082192
AGGACGT14300.08.5042525
GTATAAT9450.08.4816741
Produced by FastQC (version 0.11.5)