FastQCFastQC Report
Thu 26 May 2016
SRR1513221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6065478
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT138290.22799522148130782No Hit
GGTATCAACGCAGAGTACTTTTTTT93040.15339269221650792No Hit
TATCAACGCAGAGTACTTTTTTTTT82080.13532321772496744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.015.2914261
GTATCAA59700.011.5550891
TAGATCG752.4138519E-411.2161215
CGCTAGA1007.4027973E-49.9336781
GTGGTAT10600.09.8920281
GTATTAG4700.09.6283871
TGGTATC10050.09.4449572
GTCCTAC5800.08.9441171
GGACCGC2750.08.8369456
GTGTAGG7900.08.8019931
ATTATAC6050.08.6515533
GTATTAA7550.08.4790771
GTATTAT6900.08.4780191
GTAGGAC7300.08.4505023
GTATAAT7850.08.4362451
GTATTAC4450.08.4330851
TCTTATA6950.08.4170262
AATGCGA1000.0033676938.41202210
CTTATAC5900.08.3962493
TTATACT8400.08.3453294
Produced by FastQC (version 0.11.5)