FastQCFastQC Report
Thu 26 May 2016
SRR1513221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6065478
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT194700.3209969601736252No Hit
GGTATCAACGCAGAGTACTTTTTTT130840.21571259511616397No Hit
TATCAACGCAGAGTACTTTTTTTTT119920.19770906761181886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35850.014.3971151
CCGTTCG600.005894258611.0794739
TATACCG1856.002665E-1110.2708425
GTATCAA75200.010.0098261
GTCCTAA5350.09.9862321
GTGGTAT11100.09.3685181
ATACCGT1951.6971171E-99.2531856
ACTACGG950.00183084338.9968648
GTATTAT7500.08.904391
GTATAAG6650.08.8948251
TAGACAG10150.08.892125
GTATTAC3900.08.8065391
GTATTAA10000.08.7771841
TAGTACC3850.08.6368434
CTAATAC5400.08.4449133
TACCGTA1701.6220165E-68.3794337
ATAACCG1252.744068E-48.3604645
CTTATAC5400.08.2689773
TGGTATC9900.08.2529842
TAGGACT6000.08.233794
Produced by FastQC (version 0.11.5)