FastQCFastQC Report
Thu 26 May 2016
SRR1513220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31907
Sequences flagged as poor quality0
Sequence length25
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1930.604882941047419No Hit
TATCAACGCAGAGTACTTTTTTTTT1300.40743410536872793No Hit
GGTATCAACGCAGAGTACTTTTTTT1260.3948976713573824No Hit
ACGCAGAGTACTTTTTTTTTTTTTT460.14416899113047293No Hit
GNATCAACGCAGAGTACTTTTTTTT450.14103488262763658No Hit
GAGTACTTTTTTTTTTTTTTTTTTT390.12223023161061836No Hit
GTACTTTTTTTTTTTTTTTTTTTTT340.10655968909643652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA502.236133E-517.6935061
TATCAAC651.2106386E-515.3116882
ATCAACG602.8292941E-514.0137153
TCAACGC656.0060447E-512.9357384
CAACGCA701.2015247E-412.0117565
AACGCAG804.1521134E-410.5102866
ACGCAGA857.259033E-49.8920347
CAGAGTA900.00122586479.34247710
AGAGTAC900.00122586479.34247711
CGCAGAG950.00200719258.8507678
GCAGAGT1000.00319666148.4082299
Produced by FastQC (version 0.11.5)