FastQCFastQC Report
Thu 26 May 2016
SRR1513220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31907
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT3130.9809759613877832No Hit
TATCAACGCAGAGTACTTTTTTTTT1990.6236875920644372No Hit
GGTATCAACGCAGAGTACTTTTTTT1860.5829441815275646No Hit
GTACTTTTTTTTTTTTTTTTTTTTT850.2663992227410913No Hit
ACGCAGAGTACTTTTTTTTTTTTTT770.24132635471840036No Hit
GAGTACTTTTTTTTTTTTTTTTTTT620.19431472717585482No Hit
GCAGAGTACTTTTTTTTTTTTTTTT320.10029147209076378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA800.00410867769.5559941
Produced by FastQC (version 0.11.5)