##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513220_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31907 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.440843702008962 33.0 31.0 34.0 26.0 34.0 2 30.6056351270881 34.0 31.0 34.0 26.0 34.0 3 30.7309367850315 34.0 31.0 34.0 26.0 34.0 4 34.08875795280033 37.0 35.0 37.0 30.0 37.0 5 33.57244491804306 37.0 35.0 37.0 28.0 37.0 6 33.46243770959351 37.0 35.0 37.0 28.0 37.0 7 33.278810292412324 37.0 35.0 37.0 26.0 37.0 8 33.19318644811484 37.0 35.0 37.0 26.0 37.0 9 34.67279907230388 39.0 37.0 39.0 26.0 39.0 10 34.504246717021346 39.0 35.0 39.0 25.0 39.0 11 34.44460463221237 39.0 35.0 39.0 25.0 39.0 12 34.30146989688783 39.0 35.0 39.0 23.0 39.0 13 34.25781176544332 39.0 35.0 39.0 23.0 39.0 14 35.345472780267656 40.0 36.0 41.0 19.0 41.0 15 35.25417619958003 40.0 36.0 41.0 19.0 41.0 16 35.18190365750462 40.0 36.0 41.0 18.0 41.0 17 35.150061115115804 40.0 36.0 41.0 17.0 41.0 18 35.07747516219011 40.0 36.0 41.0 17.0 41.0 19 35.082646441219794 40.0 36.0 41.0 18.0 41.0 20 35.031685836963675 40.0 36.0 41.0 18.0 41.0 21 34.925408217632494 40.0 36.0 41.0 11.0 41.0 22 34.82151252076347 40.0 36.0 41.0 10.0 41.0 23 34.83069545867678 40.0 36.0 41.0 2.0 41.0 24 34.73178299432726 40.0 36.0 41.0 2.0 41.0 25 34.44266148494061 40.0 36.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22143653116552997 1101 2 0.17167113002987833 1101 3 -0.6346218221966708 1101 4 -0.29961164030017784 1101 5 -1.5888533846320598 1101 6 -2.7275619698988187 1101 7 -3.159002514316313 1101 8 -2.711547194810205 1101 9 -2.1735861190725245 1101 10 -1.4122795936703412 1101 11 -1.426142433313835 1101 12 -2.416915094503743 1101 13 -2.651472309704353 1101 14 -3.284969867854464 1101 15 -3.663104927297695 1101 16 -2.4840656154820024 1101 17 -3.299686228230989 1101 18 -2.339036200786232 1101 19 -2.3365179387574457 1101 20 -2.6525028268761233 1101 21 -3.3262574165552508 1101 22 -3.382254419087392 1101 23 -3.0888669265202324 1101 24 -2.031677227629608 1101 25 -3.4285579993650046 1102 1 2.1852374361429057 1102 2 1.7576439807086075 1102 3 1.973975462871202 1102 4 1.7569494456726673 1102 5 2.2595629049607027 1102 6 2.4443837314586503 1102 7 2.524255404235724 1102 8 2.7703532576784795 1102 9 2.790214785904851 1102 10 2.567358415379431 1102 11 2.9200114128400116 1102 12 2.4518631860392475 1102 13 2.653957554549038 1102 14 3.5408219873491547 1102 15 3.2101982401231197 1102 16 2.8281515790881357 1102 17 3.7138884324024914 1102 18 3.6360769213857083 1102 19 3.724568034093238 1102 20 3.3904835984632413 1102 21 2.5945570630827604 1102 22 3.3213654904148697 1102 23 2.6486896345657414 1102 24 3.782802410379439 1102 25 3.985469149956266 1103 1 -0.9686087177032476 1103 2 0.41149013455476435 1103 3 0.32012930902504877 1103 4 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2.2896900406265956 1104 17 2.060042278556338 1104 18 2.1360769213857083 1104 19 2.0322603417855447 1104 20 1.159714367694015 1104 21 2.2484032169289137 1104 22 2.3213654904148697 1104 23 1.5717665576426612 1104 24 1.667417794994826 1104 25 2.908546073033186 1105 1 0.7621605130659823 1105 2 0.41149013455476435 1105 3 0.35859084748658887 1105 4 0.5646417533649739 1105 5 -0.35582171042391764 1105 6 0.05976834684326349 1105 7 0.21656309654341754 1105 8 -0.4604159730907469 1105 9 -0.17132367563360873 1105 10 0.2596661076871243 1105 11 0.6892421820707781 1105 12 0.8364785706546343 1105 13 0.7693421699336582 1105 14 0.694668141195308 1105 15 0.6332751632000466 1105 16 0.6358438867804423 1105 17 -0.24765002913596845 1105 18 0.020692306001095062 1105 19 -0.6985088889836888 1105 20 0.04432975230939462 1105 21 0.6330186015443005 1105 22 1.2059808750302565 1105 23 1.6486896345657414 1105 24 1.0904947180717457 1105 25 0.6777768422639596 1106 1 -1.1125293132367489 1106 2 -0.8254825701598776 1106 3 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15 1.2486597785846598 1107 16 1.4297999307364861 1107 17 1.3347675532816154 1107 18 1.0097032950120877 1107 19 1.6613812209064207 1107 20 1.1185055764852194 1107 21 2.0203812389069356 1107 22 1.4916951607445412 1107 23 1.6569313928075005 1107 24 1.714121091698125 1107 25 1.9579966224837335 1108 1 1.0057502566557268 1108 2 0.6607778838425133 1108 3 0.6420666309623719 1108 4 0.6415648302880541 1108 5 0.5843492297470263 1108 6 1.1765774636523787 1108 7 0.1481870281673494 1108 8 0.3472763346015597 1108 9 0.8329498286398902 1108 10 0.5844524324734479 1108 11 0.7034871963157912 1108 12 0.8521480863241493 1108 13 0.675325075916561 1108 14 0.9339843805115464 1108 15 0.5819931119179955 1108 16 1.1771544280909794 1108 17 1.1469368654509253 1108 18 1.1816609669697584 1108 19 0.7843970939222942 1108 20 1.656865364845011 1108 21 1.5560955246212202 1108 22 1.3726475416969208 1108 23 1.2085186943947974 1108 24 1.710152837729865 1108 25 1.84159450608162 1109 1 0.17779326740841483 1109 2 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1.3467931990398228 2314 22 1.3106320378209517 2314 23 1.2059347150666326 2314 24 1.774752320934006 2314 25 0.40317934673623057 2315 1 0.09584034674581687 2315 2 -0.6685514454868162 2315 3 -0.47404948515374556 2315 4 -0.08816489944167216 2315 5 -1.1874225420247484 2315 6 -0.678277391202478 2315 7 -1.0349961550158326 2315 8 -1.2473182599930368 2315 9 -1.218101222411157 2315 10 0.05592390394491531 2315 11 -0.2234397306111333 2315 12 -0.41612018194412315 2315 13 -0.9583085577170678 2315 14 -0.8437933972662321 2315 15 -0.5080969781720981 2315 16 -1.8298525789160252 2315 17 -1.0532633347492748 2315 18 -0.7412619559531635 2315 19 -0.7681554586302539 2315 20 -1.0658573578777109 2315 21 -1.0384990699733763 2315 22 -0.8615866925373084 2315 23 -1.5602500743739682 2315 24 -0.8273846998076664 2315 25 -0.9261732616861451 2316 1 -0.8881891372836677 2316 2 -1.0850133619487323 2316 3 -2.2830175441218046 2316 4 -1.0857078969846725 2316 5 -2.5166608712630776 2316 6 -2.3545673174924 2316 7 -2.0841362041558824 2316 8 -2.8345418472166237 2316 9 -2.828666332976269 2316 10 -3.9256485776275625 2316 11 -3.6306878878592883 2316 12 -3.149535415359356 2316 13 -2.9963920958006085 2316 14 -4.093793397266232 2316 15 -5.0240674941426136 2316 16 -4.1578624069258545 2316 17 -4.252894784380725 2316 18 -4.474062938754148 2316 19 -4.387320077794875 2316 20 -4.076299618319975 2316 21 -4.171177202651506 2316 22 -3.27129185224247 2316 23 -3.463198477322372 2316 24 -2.9741905966135675 2316 25 -3.1946007801136673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1254.0 3 0.0 4 29.0 5 195.0 6 256.0 7 160.0 8 122.0 9 105.0 10 113.0 11 85.0 12 83.0 13 67.0 14 74.0 15 63.0 16 71.0 17 66.0 18 72.0 19 64.0 20 91.0 21 114.0 22 141.0 23 140.0 24 172.0 25 188.0 26 222.0 27 232.0 28 262.0 29 293.0 30 372.0 31 457.0 32 601.0 33 779.0 34 945.0 35 1332.0 36 2020.0 37 3973.0 38 16694.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.942162298387096 18.756300403225808 13.268649193548388 24.032888104838708 2 15.291315385338642 20.613031623154793 39.402012097658826 24.693640893847746 3 18.196633967467953 25.658946312721348 29.65806876234055 26.486350957470144 4 12.730748738521328 16.85523552825399 38.49625474033911 31.917760992885576 5 13.474865941233654 36.896735551444074 35.720781460691775 13.907617046630499 6 31.240793556272916 37.317829943272635 17.466386686307082 13.974989814147365 7 26.677531576143167 32.81098191619394 22.606324630958724 17.90516187670417 8 26.003698248033345 34.54414391826245 20.672579684708683 18.77957814899552 9 26.301438555802804 15.197292130253551 19.8984548845081 38.60281442943555 10 15.388472748926569 28.216378851035824 33.538095088851975 22.85705331118563 11 33.47227881029241 23.42746105870185 23.957125395681196 19.143134735324537 12 22.562447111919013 25.69028739774971 30.78634782336164 20.96091766696963 13 28.589337762873352 21.769517660701414 26.191744758203527 23.449399818221707 14 21.988905255899958 20.816748675839158 28.025198232362804 29.16914783589808 15 23.64371454539756 28.940357915191022 24.355157175541418 23.06077036387 16 22.25530447864105 28.56113078634782 26.567837778543897 22.615726956467235 17 21.66922618861065 28.128623812956405 27.257341649167895 22.944808349265053 18 21.799091051559316 26.594577652405576 29.55963015201379 22.046701144021313 19 22.957344783276397 26.22621995173473 29.720750932397277 21.095684332591595 20 22.44648509731407 26.30457266430564 29.015576519259096 22.233365719121196 21 22.562447111919013 25.828188171874512 29.551509073244116 22.05785564296236 22 23.173598269972107 25.718494374275235 28.855736985614445 22.252170370138213 23 22.546776569404834 25.589995925658947 29.316450935531392 22.546776569404834 24 22.026514557933996 26.871846303319018 28.890212179145642 22.21142695960134 25 22.556579524794685 25.791486427183248 29.29596890477086 22.355965143251208 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 32.0 1 32.0 2 29.5 3 27.0 4 27.0 5 27.0 6 77.0 7 127.0 8 127.0 9 127.0 10 131.5 11 136.0 12 136.0 13 136.0 14 180.0 15 224.0 16 224.0 17 224.0 18 327.5 19 431.0 20 431.0 21 431.0 22 622.5 23 814.0 24 814.0 25 814.0 26 1182.5 27 1551.0 28 1551.0 29 1551.0 30 1826.0 31 2101.0 32 2101.0 33 2101.0 34 2401.0 35 2701.0 36 2701.0 37 2701.0 38 2984.5 39 3268.0 40 3268.0 41 3268.0 42 3498.5 43 3729.0 44 3729.0 45 3729.0 46 3836.0 47 3943.0 48 3943.0 49 3943.0 50 3970.5 51 3998.0 52 3998.0 53 3998.0 54 3660.0 55 3322.0 56 3322.0 57 3322.0 58 2857.0 59 2392.0 60 2392.0 61 2392.0 62 1979.5 63 1567.0 64 1567.0 65 1567.0 66 1211.5 67 856.0 68 856.0 69 856.0 70 645.5 71 435.0 72 435.0 73 435.0 74 305.0 75 175.0 76 175.0 77 175.0 78 115.5 79 56.0 80 56.0 81 56.0 82 36.5 83 17.0 84 17.0 85 17.0 86 10.0 87 3.0 88 3.0 89 3.0 90 2.5 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.510859685962328 2 0.0 3 0.0 4 0.0 5 0.05641395305105463 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.006268217005672736 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.015670542514181842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 31907.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.9764628451437 #Duplication Level Percentage of deduplicated Percentage of total 1 96.89543585249106 90.08994891403141 2 2.6933189509876625 5.008305387532516 3 0.21573518506033842 0.6017488325445827 4 0.053933796265084606 0.20058294418152756 5 0.03033776039911009 0.14103488262763658 6 0.003370862266567788 0.01880465101701821 7 0.020225173599406728 0.13163255711912744 8 0.003370862266567788 0.025072868022690945 9 0.013483449066271152 0.11282790610210926 >10 0.05056293399851682 0.7803930172062556 >50 0.010112586799703364 0.7020403046353465 >100 0.010112586799703364 2.1876077349797853 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 313 0.9809759613877832 No Hit TATCAACGCAGAGTACTTTTTTTTT 199 0.6236875920644372 No Hit GGTATCAACGCAGAGTACTTTTTTT 186 0.5829441815275646 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 85 0.2663992227410913 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 77 0.24132635471840036 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 62 0.19431472717585482 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 32 0.10029147209076378 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 80 0.0041086776 9.555994 1 >>END_MODULE