FastQCFastQC Report
Thu 26 May 2016
SRR1513219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7279914
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT165520.227365323271676No Hit
GGTATCAACGCAGAGTACTTTTTTT112150.15405401767108787No Hit
TATCAACGCAGAGTACTTTTTTTTT99720.1369796401441006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34650.015.0403731
AATAGCG550.003468602711.895475
GTATCAA73400.011.3872711
GTGGTAT12100.010.2200381
GCGTTAG1152.479501E-49.6011231
GTATTAC4350.09.1376211
GTCCTAA5700.09.1042221
TGGTATC11600.08.852072
CGCTTAG1150.0026411318.6410111
CTTATAC7500.08.5987253
GTCAACG2851.5825208E-108.5231022
TCTATAC6400.08.4702123
TAAACCG1555.247064E-68.4419465
GTCTTAG8550.08.3939641
GTCTTAT6600.08.36461451
GTATTGA7700.08.3168171
CGTTATA1200.00388061588.280971
GTCTTAC6000.08.2809691
GTATAAG8200.08.2136441
TATCAAC101100.08.0379722
Produced by FastQC (version 0.11.5)