FastQCFastQC Report
Thu 26 May 2016
SRR1513219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7279914
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT235220.3231082125420713No Hit
GGTATCAACGCAGAGTACTTTTTTT157120.21582672542560258No Hit
TATCAACGCAGAGTACTTTTTTTTT144500.19849135580447794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43800.014.3769371
CCGTTTA701.0958717E-412.2096149
CGAGCGT700.001497230510.853065510
GTATCAA94500.010.4194411
CGATCGA750.002657844510.12952710
CTAATAC4650.09.3983223
GTCCTAA7650.09.1045391
TCTATAC6850.09.0150523
GTCTTAT6650.08.6084741
TTAGGAC7650.08.5690583
GTATAAG9600.08.4478121
GTGTTAG7200.08.3484271
TAATACA9250.08.3193374
TAAAGGT9050.08.2932354
GTATTAG5800.08.225051
TGGTATC12250.08.2208212
TAGGACG4400.08.2049584
GTGGTAT14000.08.178051
GTATACG2002.1557389E-78.10989951
CGTATAC2002.3081157E-78.0754063
Produced by FastQC (version 0.11.5)