FastQCFastQC Report
Thu 26 May 2016
SRR1513218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8687817
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT145890.1679248078084518No Hit
GGTATCAACGCAGAGTACTTTTTTT93410.1075183788977139No Hit
TATCAACGCAGAGTACTTTTTTTTT92620.1066090595600713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38100.014.4049671
GTATCAA77100.011.7733021
GTGGTAT14550.09.259291
GTATACG1951.3769568E-79.0607931
GTATTAA11100.09.0531421
GTAGGAC18650.08.7198683
GTATAAA13450.08.6208061
GTCCTAC16200.08.5888771
GTCCTAA10750.08.5261011
CCCTATA4300.08.4747392
TTCAACG2757.057679E-108.4327152
GTATTAC6450.08.3891361
TAGGACT10050.08.3698174
GTCTTAG9900.08.3657881
GTCTTAA8200.08.3494661
GTCCTAG9750.08.2679741
TTAGACA8850.08.2374124
CTTAGAC7200.08.1780083
TATCAAC110950.08.0519692
GTATTAT9900.08.03115751
Produced by FastQC (version 0.11.5)