FastQCFastQC Report
Thu 26 May 2016
SRR1513218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8687817
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT203480.23421303648546005No Hit
TATCAACGCAGAGTACTTTTTTTTT133450.15360590583342168No Hit
GGTATCAACGCAGAGTACTTTTTTT132930.15300736652256833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47050.013.686131
GTATCAA96050.010.0611711
GTCCTAA11950.09.1805021
GTGGTAT15650.09.143521
GTATAAT12950.08.5452491
TGGTATC14600.08.5246862
TATAACG2053.864261E-88.3421612
GTCTACG2201.3624231E-88.2388651
TAGGACG13150.08.1641814
GTATAAG7950.08.1597761
TAGACAG11100.08.131315
TTATACT10950.08.0691684
GTCCTAC17150.08.0656681
GTAGGAC16850.08.0629883
CTAGACA8650.08.0180114
TGGACTG16900.07.9829165
GTCCTAG12000.07.9497821
GTCTTAC6500.07.9253211
TAGGACA12350.07.92374044
ACTAGAC5400.07.91732933
Produced by FastQC (version 0.11.5)