FastQCFastQC Report
Thu 26 May 2016
SRR1513217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6057550
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT160960.26571798829559806No Hit
GGTATCAACGCAGAGTACTTTTTTT116640.19255309489810238No Hit
TATCAACGCAGAGTACTTTTTTTTT100750.16632136754958687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32550.015.1635081
GTATCAA64050.010.9470661
GTGGTAT10150.010.3347681
ATACCGT1608.08177E-99.9305126
GTCCTAC6750.09.8150071
GCGTTAT1152.4779825E-49.6016381
GTATTAG4900.09.2391271
TAACCCG1358.429402E-69.0002025
TATACCG1055.2912906E-48.90129855
GGTCTAC6450.08.7307911
CCCCTAT2456.833943E-98.5630931
ACCGTGC3400.08.5216148
GTCAACG3253.6379788E-128.4937562
CTTATAC5300.08.4646323
CGTGCTA1000.00336900548.41165810
TCTTATA5650.08.4035572
TTATACT8900.08.4012274
CGTTTAA1856.019538E-68.356021
CCGTATA1358.233102E-58.3078119
GCGTGCG2151.3025783E-88.2595099
Produced by FastQC (version 0.11.5)