FastQCFastQC Report
Thu 26 May 2016
SRR1513217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6057550
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT232730.3841982319584651No Hit
GGTATCAACGCAGAGTACTTTTTTT161170.2660646631063714No Hit
TATCAACGCAGAGTACTTTTTTTTT144200.23805003673102162No Hit
ACGCAGAGTACTTTTTTTTTTTTTT65960.10888890723147147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42250.014.1365871
GTATCAA86850.010.2276731
GTCTACG1606.2198524E-89.5410671
GTGGTAT13200.09.2519441
GCGCTAT850.0071812758.9798281
GTCCTAA7000.08.8595631
TGGTATC12600.08.8221762
ATAGGAC5550.08.730473
GTATTAA8350.08.6840851
GTCCTAC8500.08.6430851
AAGGCGT4200.08.5920656
TAGGACG6350.08.5282814
TAAAGGT8400.08.3697584
TTAGGAC7750.08.336193
TTATACT8100.08.3278664
CGTATAC1150.00111047768.2615693
ACACCGT3350.08.2208446
GTTCTAA5600.08.1780581
GTCTTAG6300.08.1780581
GTATAAG7500.08.141711
Produced by FastQC (version 0.11.5)