FastQCFastQC Report
Thu 26 May 2016
SRR1513216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7930626
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87930.11087397136115107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31700.014.1513841
GTATCAA62300.010.8896141
GTAGGAC15000.010.0934283
GTATAAA8400.09.8653871
TAGGACG10650.09.6529044
GACCGCG1952.237357E-109.58534057
GTCTTAT6000.09.3918491
TAGGACC9400.09.3457664
GTGGTAT13500.09.0849251
TTAGGAC9750.09.0102773
GCGTACA2851.6370905E-118.9169251
GTCCTAC12950.08.7881941
GTTCTAT8400.08.68154051
GCACCGT4650.08.6423226
GTCCTAG8750.08.5868331
CTTACAC9600.08.5669533
CTTATAC7450.08.5303643
GTATTAA7950.08.4780281
GTATTAC6400.08.459571
GTATAAT10500.08.4184641
Produced by FastQC (version 0.11.5)