##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513216_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7930626 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.44214567677255 33.0 31.0 34.0 25.0 34.0 2 24.688818890211188 31.0 16.0 34.0 2.0 34.0 3 27.672833266882083 31.0 22.0 34.0 16.0 34.0 4 33.319405302935735 35.0 32.0 37.0 28.0 37.0 5 34.099363782884225 37.0 35.0 37.0 31.0 37.0 6 34.424533826207416 37.0 35.0 37.0 32.0 37.0 7 34.5514911433221 37.0 35.0 37.0 32.0 37.0 8 34.6026593613165 37.0 35.0 37.0 32.0 37.0 9 36.309318457332374 39.0 37.0 39.0 32.0 39.0 10 36.23285274075464 39.0 37.0 39.0 32.0 39.0 11 36.256964330432425 39.0 37.0 39.0 32.0 39.0 12 36.164677416385544 39.0 37.0 39.0 32.0 39.0 13 36.15933937623587 39.0 37.0 39.0 32.0 39.0 14 37.49471668440802 40.0 38.0 41.0 32.0 41.0 15 37.448447323073864 40.0 38.0 41.0 32.0 41.0 16 37.400002093151286 40.0 38.0 41.0 32.0 41.0 17 37.34722996646166 40.0 38.0 41.0 32.0 41.0 18 37.31266661673366 40.0 38.0 41.0 32.0 41.0 19 37.31958095111281 40.0 38.0 41.0 32.0 41.0 20 37.26898544957233 40.0 38.0 41.0 32.0 41.0 21 37.19949774456644 40.0 38.0 41.0 32.0 41.0 22 37.12111187691867 40.0 38.0 41.0 31.0 41.0 23 37.02162288323772 40.0 38.0 41.0 31.0 41.0 24 36.937342651134976 40.0 38.0 41.0 31.0 41.0 25 36.832603756626526 40.0 38.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6850633244629414 1101 2 6.484165773248851 1101 3 3.7286337783706216 1101 4 1.4303485095602824 1101 5 0.7351597311182658 1101 6 0.4056542614891967 1101 7 0.1874817391807042 1101 8 0.07395216720605191 1101 9 0.08581731598093256 1101 10 0.00555166159104914 1101 11 0.09889461846331216 1101 12 0.021349598439904582 1101 13 0.15815862811693648 1101 14 0.13938894918328515 1101 15 0.12091600999435315 1101 16 0.17041917048339883 1101 17 0.1961306703239245 1101 18 0.14065823231660346 1101 19 0.16664965715943225 1101 20 0.10241765876494213 1101 21 0.02222718098734333 1101 22 0.10522077032089072 1101 23 0.07139021292762493 1101 24 0.08640329889273346 1101 25 0.041339560809909415 1102 1 1.1252544966297755 1102 2 7.050398761989314 1102 3 4.109421022009613 1102 4 1.9506413659531887 1102 5 1.0966403029212728 1102 6 0.7823680611455259 1102 7 0.6372398692158114 1102 8 0.5908094658691283 1102 9 0.5073189605827935 1102 10 0.5425684263681703 1102 11 0.39856535004865634 1102 12 0.6075884923171202 1102 13 0.5205774757722281 1102 14 0.6776051630041451 1102 15 0.45501025765744885 1102 16 0.5258013359614182 1102 17 0.620650361170668 1102 18 0.58985778781242 1102 19 0.5443481736137912 1102 20 0.6137981953294229 1102 21 0.6265371046563217 1102 22 0.6550478533831949 1102 23 0.6429151140371445 1102 24 0.6536860780047107 1102 25 0.4803941075287881 1103 1 1.0616551513890222 1103 2 6.6906199986669 1103 3 3.8771646037299945 1103 4 1.6337721624137416 1103 5 0.9496036241995967 1103 6 0.6202088953320768 1103 7 0.3988430963246046 1103 8 0.35789699344344683 1103 9 0.3934019918314391 1103 10 0.4186029466832295 1103 11 0.44449453274194894 1103 12 0.4497989468951502 1103 13 0.3816101300092569 1103 14 0.47422037771310244 1103 15 0.3920612015878362 1103 16 0.3195138946666063 1103 17 0.43983098262431497 1103 18 0.3959453884688813 1103 19 0.5083565837365853 1103 20 0.5450403682673155 1103 21 0.5309822550884675 1103 22 0.5079294716630969 1103 23 0.5196932629019244 1103 24 0.4552530514035169 1103 25 0.5741430653987081 1104 1 1.0175555918444346 1104 2 6.520307109113173 1104 3 3.8334182395647822 1104 4 1.7030670182269247 1104 5 0.9068302786775604 1104 6 0.7101816092576669 1104 7 0.5276084360847051 1104 8 0.4644297228819312 1104 9 0.35784906792773086 1104 10 0.4474396180591711 1104 11 0.4103786292543177 1104 12 0.5333386571364329 1104 13 0.4148506629880089 1104 14 0.4847573731977377 1104 15 0.5188645756425174 1104 16 0.4474190997969387 1104 17 0.5367803441024748 1104 18 0.5635095869231321 1104 19 0.5685099120836341 1104 20 0.4994726240084759 1104 21 0.495015192288939 1104 22 0.5537880827049548 1104 23 0.572900415454626 1104 24 0.5479585280095165 1104 25 0.6010198486891056 1105 1 0.8342334173144259 1105 2 6.369283571266532 1105 3 3.798759075499202 1105 4 1.6515053165952551 1105 5 0.9206092096822758 1105 6 0.5767662903440396 1105 7 0.4602807006757459 1105 8 0.3533523855982921 1105 9 0.2517186495268149 1105 10 0.3599079882730223 1105 11 0.2883090010322178 1105 12 0.3553911066744675 1105 13 0.3825759815899161 1105 14 0.38710776956114756 1105 15 0.4591449304111421 1105 16 0.3650524938034394 1105 17 0.3672725083185 1105 18 0.4082279717066797 1105 19 0.4170979394556795 1105 20 0.40701481173930176 1105 21 0.4281850965897789 1105 22 0.2917369478275518 1105 23 0.24924516419858378 1105 24 0.15418327669926413 1105 25 0.3011690355647261 1106 1 0.5547558657564693 1106 2 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0.7291130142910234 1107 14 0.9238235136788759 1107 15 0.902336585778805 1107 16 0.9001941524244117 1107 17 0.8135654726067969 1107 18 0.7054316424417735 1107 19 0.8467211438150386 1107 20 0.8157101591539941 1107 21 0.9626587201992791 1107 22 1.0047993980897658 1107 23 1.0545855766097816 1107 24 1.0694684069873546 1107 25 1.035196374962311 1108 1 1.247112609148683 1108 2 7.055677297507714 1108 3 4.181737242013504 1108 4 1.9257192956896318 1108 5 1.1572649767988707 1108 6 0.9419931822134444 1108 7 0.6722868936324886 1108 8 0.6035878047332517 1108 9 0.6259920266272587 1108 10 0.5775621889603926 1108 11 0.6601775658137043 1108 12 0.5938860510568844 1108 13 0.6570914640330514 1108 14 0.7449813886438363 1108 15 0.7285219656143127 1108 16 0.7301121535477861 1108 17 0.7848161634766271 1108 18 0.743690229371829 1108 19 0.77284655486703 1108 20 0.7786463760147839 1108 21 0.7333999146485084 1108 22 0.7291188087255023 1108 23 0.7827505358852349 1108 24 0.7821880141791908 1108 25 0.8515300104319365 1109 1 1.0826354951672919 1109 2 6.820640970786318 1109 3 3.9392400211162943 1109 4 1.7691615858860672 1109 5 0.9865508786338992 1109 6 0.6411467465808087 1109 7 0.44807343324924176 1109 8 0.4186188181549042 1109 9 0.41485386561491566 1109 10 0.34282642997406754 1109 11 0.35033519946344427 1109 12 0.3563818621568231 1109 13 0.25451428879424043 1109 14 0.28603946919540135 1109 15 0.31455482770840604 1109 16 0.437699326582603 1109 17 0.35895947455073696 1109 18 0.4228143744052275 1109 19 0.42140247566849354 1109 20 0.4195729504423866 1109 21 0.4799453002950358 1109 22 0.4369827106904296 1109 23 0.40689478715007965 1109 24 0.48029211711615716 1109 25 0.5226517146845566 1110 1 0.8496360172179891 1110 2 6.679597371505032 1110 3 3.77764875389769 1110 4 1.5593787990963293 1110 5 0.7758615903309831 1110 6 0.5182140781473805 1110 7 0.2720771524700538 1110 8 0.17854724099242958 1110 9 0.23326085542447572 1110 10 0.24291645549040908 1110 11 0.20460978083188053 1110 12 0.2525735047330002 1110 13 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-0.8722565081087907 2315 1 -1.5571496342068087 2315 2 -2.9782203235316302 2315 3 -0.3554431906315294 2315 4 -0.7398833694507445 2315 5 -0.8210303093074671 2315 6 -0.7503737075266486 2315 7 -0.7949666249104936 2315 8 -0.8538824032240129 2315 9 -0.865210698684237 2315 10 -0.9878390496841405 2315 11 -0.9257011159984714 2315 12 -0.9812590567374784 2315 13 -0.9624720385132122 2315 14 -1.066822424150594 2315 15 -1.0189877204685516 2315 16 -1.0738308893498214 2315 17 -1.7551948801254227 2315 18 -1.3179879211733834 2315 19 -1.2426887846595278 2315 20 -1.2146394074572342 2315 21 -1.213490816063711 2315 22 -1.0849405038561315 2315 23 -1.0841190248655863 2315 24 -1.0318382776654218 2315 25 -1.1084607804514093 2316 1 -0.06367061057796164 2316 2 4.971997040921803 2316 3 2.699773663171115 2316 4 0.6434027308229346 2316 5 -0.12821817251532508 2316 6 -0.38800296975001913 2316 7 -0.5889699893892484 2316 8 -0.593384658983247 2316 9 -0.6917851582412098 2316 10 -0.7245575529658197 2316 11 -0.7484463361531155 2316 12 -0.825898151511872 2316 13 -0.748179337757314 2316 14 -1.0214745811497963 2316 15 -0.9298057034851794 2316 16 -0.965606067170917 2316 17 -0.9935026236100484 2316 18 -0.9340957759234243 2316 19 -1.0141322900881633 2316 20 -1.0512634873876863 2316 21 -1.0141182707372494 2316 22 -0.9060227302662014 2316 23 -0.9858067300026434 2316 24 -0.9649477928431267 2316 25 -0.923511895996711 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 203943.0 3 4.0 4 1928.0 5 8044.0 6 9431.0 7 8905.0 8 8362.0 9 8135.0 10 7979.0 11 7904.0 12 7705.0 13 7788.0 14 7917.0 15 7805.0 16 8484.0 17 9123.0 18 10591.0 19 12849.0 20 16595.0 21 20666.0 22 25378.0 23 30079.0 24 34450.0 25 39708.0 26 44934.0 27 51848.0 28 59224.0 29 72309.0 30 92092.0 31 119019.0 32 157727.0 33 220595.0 34 329729.0 35 579441.0 36 1315489.0 37 963830.0 38 3420616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.9228472002084 17.482339981736626 12.374294286478772 25.220518531576197 2 18.608463401478915 20.103492989006682 36.80554582406938 24.48249778544503 3 19.06377882401717 23.6559005556434 28.90776844097805 28.372552179361378 4 13.17969098530179 15.779813598573428 36.41759175126907 34.62290366485571 5 14.605087190278246 36.72442364284304 34.43929616298216 14.231193003896559 6 33.841570904512906 35.24782823389251 17.180784554446856 13.729816307147724 7 29.5749036709082 30.89288285691445 21.074553257208194 18.45766021496916 8 27.058368406226695 34.394699737448214 19.639193677775246 18.90773817854984 9 26.9920432510624 15.09558009670359 18.890942530892264 39.02143412134175 10 15.847828405979552 27.932839097443253 32.97728325607587 23.242049240501316 11 35.600906057329155 21.96291237612576 22.998770336356884 19.437411230188193 12 23.784679797029895 23.899046556980498 30.09456000068595 22.221713645303662 13 29.15695784820765 20.710887894276595 25.35489227987373 24.777261977642027 14 22.683593955886963 20.049993531405967 26.379166033047074 30.887246479659993 15 24.42746638159459 28.026160860441536 22.84397221606466 24.702400541899213 16 24.49942072566405 26.952119205932657 24.773863605986524 23.77459646241677 17 23.592733050246363 27.001692847482957 25.881071947594236 23.52450215467645 18 23.5615183945881 26.725151493447473 26.50821348193326 23.20511663003116 19 24.55498091888208 26.206441745060125 26.171740779246 23.066836556811797 20 24.708001322976656 25.884419100141336 26.218693892177907 23.188885684704097 21 24.790766832277804 25.58547080646597 25.932454764604962 23.69130759665126 22 24.818962631193696 25.529683016740968 25.891978420146412 23.759375931918928 23 24.470012709732977 25.609739950464956 25.94027623461519 23.97997110518688 24 24.275071435245387 25.70076270309127 26.062235573585834 23.9619302880775 25 24.354647413709838 25.63872007077373 26.021085851230406 23.985546664286023 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 563.0 1 563.0 2 800.5 3 1038.0 4 1038.0 5 1038.0 6 2700.0 7 4362.0 8 4362.0 9 4362.0 10 6310.0 11 8258.0 12 8258.0 13 8258.0 14 14458.0 15 20658.0 16 20658.0 17 20658.0 18 39490.5 19 58323.0 20 58323.0 21 58323.0 22 93209.0 23 128095.0 24 128095.0 25 128095.0 26 186130.0 27 244165.0 28 244165.0 29 244165.0 30 322509.5 31 400854.0 32 400854.0 33 400854.0 34 489911.0 35 578968.0 36 578968.0 37 578968.0 38 676693.5 39 774419.0 40 774419.0 41 774419.0 42 859526.0 43 944633.0 44 944633.0 45 944633.0 46 1004345.0 47 1064057.0 48 1064057.0 49 1064057.0 50 1079051.0 51 1094045.0 52 1094045.0 53 1094045.0 54 1020883.5 55 947722.0 56 947722.0 57 947722.0 58 830196.5 59 712671.0 60 712671.0 61 712671.0 62 594921.0 63 477171.0 64 477171.0 65 477171.0 66 372560.0 67 267949.0 68 267949.0 69 267949.0 70 197129.5 71 126310.0 72 126310.0 73 126310.0 74 88566.5 75 50823.0 76 50823.0 77 50823.0 78 34341.0 79 17859.0 80 17859.0 81 17859.0 82 11680.5 83 5502.0 84 5502.0 85 5502.0 86 3553.5 87 1605.0 88 1605.0 89 1605.0 90 1013.0 91 421.0 92 421.0 93 421.0 94 269.5 95 118.0 96 118.0 97 118.0 98 77.5 99 37.0 100 37.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 15.417849738469574 3 0.0 4 0.0 5 5.04373803530768E-4 6 0.0026983998488896085 7 0.0 8 0.0 9 0.0 10 0.0 11 2.5218690176538396E-5 12 0.0 13 0.0015257307556805731 14 0.0 15 0.0 16 1.7653083123576877E-4 17 0.0025344783627421087 18 0.0031523362720673 19 1.387027959709612E-4 20 3.1523362720672996E-4 21 0.0 22 0.001021356952149805 23 0.006090313677634023 24 2.1435886650057637E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 7930626.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.14738616331384 #Duplication Level Percentage of deduplicated Percentage of total 1 73.45820672512168 42.71402713310189 2 15.554871232245032 18.089502085239467 3 4.859481972351851 8.476985244000153 4 2.1099691924212856 4.907567736976637 5 1.1319204820834305 3.2909108688934796 6 0.7076321912387042 2.468817737132933 7 0.4623006475336764 1.8817101992983503 8 0.3212722104611803 1.494491142818215 9 0.22153065804105432 1.159328584811359 >10 1.1088085957576324 11.113012366565854 >50 0.04638348771087589 1.8337750533136694 >100 0.016690278695983964 1.6056751015612332 >500 3.9963733988573045E-4 0.156163119053754 >1k 4.661028768321837E-4 0.5535009114104131 >5k 6.658612082591433E-5 0.2545327158225767 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8793 0.11087397136115107 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.5218690176538396E-5 0.0 0.0 0.0 0.0 3 2.5218690176538396E-5 0.0 0.0 0.0 0.0 4 2.5218690176538396E-5 0.0 0.0 0.0 0.0 5 2.5218690176538396E-5 1.2609345088269198E-5 0.0 0.0 1.2609345088269198E-5 6 3.782803526480759E-5 1.2609345088269198E-5 0.0 0.0 1.2609345088269198E-5 7 3.782803526480759E-5 3.782803526480759E-5 0.0 0.0 1.2609345088269198E-5 8 3.782803526480759E-5 3.782803526480759E-5 0.0 0.0 1.2609345088269198E-5 9 3.782803526480759E-5 3.782803526480759E-5 0.0 1.2609345088269198E-5 1.2609345088269198E-5 10 3.782803526480759E-5 3.782803526480759E-5 0.0 2.5218690176538396E-5 1.2609345088269198E-5 11 3.782803526480759E-5 3.782803526480759E-5 0.0 3.782803526480759E-5 1.2609345088269198E-5 12 3.782803526480759E-5 3.782803526480759E-5 0.0 3.782803526480759E-5 2.5218690176538396E-5 13 3.782803526480759E-5 3.782803526480759E-5 0.0 6.3046725441346E-5 5.043738035307679E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3170 0.0 14.151384 1 GTATCAA 6230 0.0 10.889614 1 GTAGGAC 1500 0.0 10.093428 3 GTATAAA 840 0.0 9.865387 1 TAGGACG 1065 0.0 9.652904 4 GACCGCG 195 2.237357E-10 9.5853405 7 GTCTTAT 600 0.0 9.391849 1 TAGGACC 940 0.0 9.345766 4 GTGGTAT 1350 0.0 9.084925 1 TTAGGAC 975 0.0 9.010277 3 GCGTACA 285 1.6370905E-11 8.916925 1 GTCCTAC 1295 0.0 8.788194 1 GTTCTAT 840 0.0 8.6815405 1 GCACCGT 465 0.0 8.642322 6 GTCCTAG 875 0.0 8.586833 1 CTTACAC 960 0.0 8.566953 3 CTTATAC 745 0.0 8.530364 3 GTATTAA 795 0.0 8.478028 1 GTATTAC 640 0.0 8.45957 1 GTATAAT 1050 0.0 8.418464 1 >>END_MODULE