FastQCFastQC Report
Thu 26 May 2016
SRR1513216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7930626
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT133270.1680447419913636No Hit
GGTATCAACGCAGAGTACTTTTTTT82890.10451886143666338No Hit
TATCAACGCAGAGTACTTTTTTTTT82620.10417840911928011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40750.013.063471
GTATCAA84000.010.0852261
GTCCTAC15100.09.413721
TAGGACT9300.09.1946554
GTCCTAA9550.09.0905461
GTAGGAC15550.09.042893
CGTTAGT950.00182317569.0010852
GTCCTAG9450.08.88388251
TTAGGAC10300.08.7631913
TAGGACG12100.08.7159264
ACCGTCG1201.7722328E-48.7053198
CCGATCG1107.1764877E-48.6332119
TAGGACA11700.08.5266694
TATACTG14100.08.4903855
GTCTTAT6700.08.4009651
GTATACG2053.6026904E-88.3766551
AGGACGT12700.08.2293395
TTATACT10050.08.2248734
GGACCGT4550.08.14003856
ACCGTCC3400.08.1001378
Produced by FastQC (version 0.11.5)