FastQCFastQC Report
Thu 26 May 2016
SRR1513215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6718559
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT156270.23259451915209794No Hit
GGTATCAACGCAGAGTACTTTTTTT107270.15966221328115152No Hit
TATCAACGCAGAGTACTTTTTTTTT92680.1379462471044758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35800.014.9873611
GTATCAA68550.010.7582331
GTCCTAC9400.09.7481491
TGGTATC11100.09.6476312
GTGGTAT12350.09.56508351
ATAAGAC4300.09.34663
TCGACGC1003.2472884E-49.346614
CCGATCG800.00516793229.346539
TAACCCG1055.2901544E-48.9015235
GTCCTAA6850.08.8642921
ACCGCGT950.00211635958.8546088
GTATAAG7650.08.658881
GTATACG1150.00264371148.6400571
TAGGACC7200.08.5677164
GTCTTAG7150.08.4923641
CTTATAC7550.08.4181293
GTCTTAT5000.08.3904551
ACCGTGC4250.08.3568968
TCTTATA6900.08.3200552
ATAGGAC4950.08.3080883
Produced by FastQC (version 0.11.5)