FastQCFastQC Report
Thu 26 May 2016
SRR1513215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6718559
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT221740.3300410102821155No Hit
GGTATCAACGCAGAGTACTTTTTTT153050.2278018247662929No Hit
TATCAACGCAGAGTACTTTTTTTTT137050.20398719427782058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45450.014.399451
GTATCAA92250.09.7418211
GTCCTAC10000.09.4447571
GTACTAG4000.09.3016571
GTATTAG5050.09.2567891
GTGGTAT13500.08.9041491
GTATTAT6500.08.659531
GACTGCG3000.08.5468457
GTCTTAG8150.08.4281171
TGGTATC12750.08.4201982
CTATTAG4200.08.4044271
GTATAGA8800.08.347641
CGTATAC1150.00111067668.261453
ACCGTCC3001.8189894E-128.2302968
TAGACAG10750.08.2191815
TTAGACT5450.08.1932354
ATAATAC6400.08.1646353
TACACCG3053.6379788E-128.0989285
GTATTAC4850.08.06487751
CGAATTT1304.202276E-48.03615315
Produced by FastQC (version 0.11.5)