FastQCFastQC Report
Thu 26 May 2016
SRR1513214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6881200
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT181710.26406731384060916No Hit
GGTATCAACGCAGAGTACTTTTTTT115450.16777596930767888No Hit
TATCAACGCAGAGTACTTTTTTTTT113680.16520374353310469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35550.015.3835271
GTATCAA75000.011.8583671
TATACCG1408.949428E-1011.3484885
CGCTTAA800.001290259811.0481691
CCGATCG600.006641142510.9032129
GTCCTAC19400.010.7634221
CGATCGC700.001710970110.680696510
TAGGACC12100.010.2726714
TCCTACA24700.09.9299332
TGTAGGA25500.09.4884272
TCTATAC5700.09.3458143
TTAGGAC15650.09.3159543
GTCCTAA12600.09.2944911
GTATTAG5600.09.2725711
GTCAACG3100.09.2662062
GTAGGAC24600.09.1938493
CGTGCGC3400.09.07073910
TATACTG10800.08.8266015
GTCCTAG8450.08.7600861
GTATTAA7700.08.7524461
Produced by FastQC (version 0.11.5)