##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513214_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6881200 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.51323664477126 33.0 31.0 34.0 25.0 34.0 2 24.74792027553334 31.0 16.0 34.0 2.0 34.0 3 27.738288961227692 31.0 22.0 34.0 16.0 34.0 4 33.390908998430504 35.0 32.0 37.0 28.0 37.0 5 34.17877492297855 37.0 35.0 37.0 32.0 37.0 6 34.50648113701099 37.0 35.0 37.0 32.0 37.0 7 34.63597788176481 37.0 35.0 37.0 32.0 37.0 8 34.68481107946288 37.0 35.0 37.0 32.0 37.0 9 36.39870880079056 39.0 37.0 39.0 32.0 39.0 10 36.326627477765506 39.0 37.0 39.0 32.0 39.0 11 36.34898549671569 39.0 37.0 39.0 32.0 39.0 12 36.26681160262745 39.0 37.0 39.0 32.0 39.0 13 36.26049439051328 39.0 37.0 39.0 32.0 39.0 14 37.609079811660756 40.0 38.0 41.0 32.0 41.0 15 37.56173094809045 40.0 38.0 41.0 32.0 41.0 16 37.52047956751729 40.0 38.0 41.0 32.0 41.0 17 37.47627884671278 40.0 38.0 41.0 32.0 41.0 18 37.45107190606289 40.0 38.0 41.0 32.0 41.0 19 37.463461896180895 40.0 38.0 41.0 32.0 41.0 20 37.41895120037203 40.0 38.0 41.0 32.0 41.0 21 37.34952144974714 40.0 38.0 41.0 32.0 41.0 22 37.27331584607336 40.0 38.0 41.0 32.0 41.0 23 37.180043887694005 40.0 38.0 41.0 32.0 41.0 24 37.104556472708246 40.0 38.0 41.0 32.0 41.0 25 37.00924795093879 40.0 38.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5816327246795439 1101 2 6.434940555383676 1101 3 3.6288053998205783 1101 4 1.3324252231131695 1101 5 0.5880410051761586 1101 6 0.2797136778338114 1101 7 0.08493853717237698 1101 8 0.012299198518974208 1101 9 0.013388955956955328 1101 10 -0.03252471866284168 1101 11 -0.014599553107927932 1101 12 -0.06354762172436779 1101 13 0.011280694780090528 1101 14 0.008223255591978784 1101 15 0.0010920745598355097 1101 16 0.05162964937119341 1101 17 0.07411689441943992 1101 18 0.016107246670287623 1101 19 0.05209265746835712 1101 20 -0.016690474279741352 1101 21 -0.06654576135280621 1101 22 -0.06118056408902817 1101 23 0.03356475482399901 1101 24 -0.011101236970709749 1101 25 0.0130048498355535 1102 1 1.07901525431404 1102 2 6.93520318476952 1102 3 4.010690785827276 1102 4 1.7163524406323205 1102 5 0.9662987583138616 1102 6 0.6467637258751182 1102 7 0.5134220394373656 1102 8 0.45587179609484707 1102 9 0.42601000256789234 1102 10 0.4549978549755167 1102 11 0.32759072232511244 1102 12 0.48522496972482543 1102 13 0.4862887154201232 1102 14 0.550224089296762 1102 15 0.3867460751327485 1102 16 0.5152041223337633 1102 17 0.5650851452101477 1102 18 0.5320401324886816 1102 19 0.4668944297500488 1102 20 0.5277387497405357 1102 21 0.5314536675839392 1102 22 0.5704257410260993 1102 23 0.4469329704469658 1102 24 0.5091388969987989 1102 25 0.4737308274148546 1103 1 1.0412482003928893 1103 2 6.704686652470684 1103 3 3.904531678822636 1103 4 1.666072854646032 1103 5 0.9537803191704413 1103 6 0.6074843854545193 1103 7 0.4346773849088734 1103 8 0.39633380804670537 1103 9 0.4694223370338122 1103 10 0.47087081963336885 1103 11 0.4930830552105192 1103 12 0.5282474122044079 1103 13 0.40255267902100655 1103 14 0.5165594764235877 1103 15 0.4607062196288183 1103 16 0.3848961843572951 1103 17 0.5728490564663744 1103 18 0.47126719278450935 1103 19 0.618759803166995 1103 20 0.5691576438431554 1103 21 0.5899180565639526 1103 22 0.6477380531692205 1103 23 0.6196494615153867 1103 24 0.5955523042199218 1103 25 0.6877863181111294 1104 1 1.0909114179912507 1104 2 6.607100176944829 1104 3 3.899670461262538 1104 4 1.8214878047092498 1104 5 1.0078649938821087 1104 6 0.7263442725939697 1104 7 0.5332024923002621 1104 8 0.5310598937113653 1104 9 0.37761723421437665 1104 10 0.44029356866406744 1104 11 0.46810642959219706 1104 12 0.5531438590320121 1104 13 0.4214167797242112 1104 14 0.5209102715884484 1104 15 0.49818490057025855 1104 16 0.4149316533620535 1104 17 0.5702711545741224 1104 18 0.5956325852840294 1104 19 0.5463730541953851 1104 20 0.5093504652381782 1104 21 0.5608478568967286 1104 22 0.5812338024340846 1104 23 0.659197123338636 1104 24 0.6456746791878629 1104 25 0.6918945427696315 1105 1 0.7914669572909396 1105 2 6.39131268427025 1105 3 3.8219202244459183 1105 4 1.6518911221278927 1105 5 0.8749223888769535 1105 6 0.580805479381624 1105 7 0.45018253483735293 1105 8 0.28260053257157125 1105 9 0.24630165722145136 1105 10 0.4044635576966442 1105 11 0.26671200538532247 1105 12 0.4321385743873094 1105 13 0.38339453018429737 1105 14 0.45464089043513667 1105 15 0.48450694132610295 1105 16 0.3658736376673488 1105 17 0.4611800403708699 1105 18 0.4589186822065088 1105 19 0.40594298980688137 1105 20 0.4117989393353554 1105 21 0.4147528310622377 1105 22 0.297948690264505 1105 23 0.22279510960121485 1105 24 0.18026957734525695 1105 25 0.24621535763738223 1106 1 0.5761309908348373 1106 2 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1107 14 0.9355207970493353 1107 15 0.8816452394277121 1107 16 0.8774229362883688 1107 17 0.876201126829443 1107 18 0.7028840095095532 1107 19 0.8012687684672599 1107 20 0.749301926979463 1107 21 0.8903894041784639 1107 22 0.9948131553077317 1107 23 1.0321731399236 1107 24 1.03296308570215 1107 25 1.0316794727390999 1108 1 1.1570690184633996 1108 2 6.979285416783398 1108 3 4.097287581672109 1108 4 1.8525129068778696 1108 5 1.071767228843953 1108 6 0.8209281067365524 1108 7 0.5860299561314903 1108 8 0.49281465153185877 1108 9 0.5140300007571739 1108 10 0.568336729669511 1108 11 0.5332680340630418 1108 12 0.5345145682062054 1108 13 0.6327522368777423 1108 14 0.6547535197957046 1108 15 0.6689699608424107 1108 16 0.6824315817547273 1108 17 0.7024112446685109 1108 18 0.6123275235832679 1108 19 0.6021223797604733 1108 20 0.6577576177667268 1108 21 0.5468966283802459 1108 22 0.596083782437077 1108 23 0.6465778971432954 1108 24 0.6318634120493982 1108 25 0.6476790531682539 1109 1 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-0.3533405570970949 2315 4 -0.8458593918880482 2315 5 -0.8974975325133556 2315 6 -0.8317505876552076 2315 7 -0.8251318086876083 2315 8 -0.9551384496640765 2315 9 -0.9917250509985678 2315 10 -1.0935395582363014 2315 11 -1.0356787123949758 2315 12 -1.0303530079176397 2315 13 -1.048903696705878 2315 14 -1.2017285438303702 2315 15 -1.1860401264165503 2315 16 -1.2066118308823803 2315 17 -1.7231816410873435 2315 18 -1.3459097915335505 2315 19 -1.2701705053110715 2315 20 -1.2545410219823339 2315 21 -1.2959056728919833 2315 22 -1.1788757145557796 2315 23 -1.2324452982060876 2315 24 -1.2055509627971617 2315 25 -1.2906976209489116 2316 1 -0.04225834015774055 2316 2 4.8760390403428815 2316 3 2.683854948536787 2316 4 0.6199425556019733 2316 5 -0.14671564766419465 2316 6 -0.4236719741011683 2316 7 -0.6087371476587933 2316 8 -0.6011074721616367 2316 9 -0.7550463008519301 2316 10 -0.8592996743775387 2316 11 -0.7951022665433598 2316 12 -0.8637542997674856 2316 13 -0.7780683795736394 2316 14 -1.0481039073213125 2316 15 -0.9621362369696911 2316 16 -0.9499960894059072 2316 17 -1.0216800420381986 2316 18 -0.9237993930173047 2316 19 -1.0199689888737424 2316 20 -1.027929649152739 2316 21 -0.9930462653637093 2316 22 -0.8570694592196233 2316 23 -0.9495100945471435 2316 24 -0.9720861813399537 2316 25 -0.9729798009015624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 173760.0 3 12.0 4 1582.0 5 6766.0 6 7720.0 7 7422.0 8 6944.0 9 6589.0 10 6494.0 11 6310.0 12 6352.0 13 6330.0 14 6363.0 15 6522.0 16 7039.0 17 7534.0 18 8550.0 19 10665.0 20 13572.0 21 17559.0 22 20910.0 23 24798.0 24 28637.0 25 32447.0 26 37085.0 27 42839.0 28 48263.0 29 59488.0 30 75830.0 31 97697.0 32 130360.0 33 182277.0 34 274602.0 35 485207.0 36 1151050.0 37 822017.0 38 3053608.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.251031796779635 18.139902923908622 12.765258966459339 24.843806312852408 2 18.10295288133689 20.686782032267757 37.37181655553469 23.838448530860664 3 18.59354473057025 24.476937162122887 29.641021914782307 27.28849619252456 4 12.817197581817124 16.418982154275415 37.488781026565135 33.27503923734233 5 13.948771755945396 37.29804473515749 34.89730300029137 13.855880508605736 6 32.367676023721394 36.31466257969173 17.734336068333786 13.583325328253098 7 28.319217578329358 31.705879788408996 21.853019822124047 18.121882811137592 8 26.301531709585536 34.89972679183863 20.315700749869208 18.48304074870662 9 26.74202174039412 15.22503342440272 19.778483404057432 38.25446143114573 10 15.607888159042027 28.280067430099404 33.57000232517584 22.542042085682727 11 34.352380843042276 22.622125191300295 23.869553509367627 19.155940456289798 12 23.27525722257746 24.602104284136487 30.79217287682381 21.330465616462245 13 28.571530299172576 21.568054698136 25.88985617440902 23.970558828282407 14 22.129991861884555 21.008443294774167 27.275649596000694 29.585915247340584 15 23.657617857350463 28.95612683834215 23.65944893332558 23.726806370981805 16 23.367065410972092 27.73525199511421 25.939819958335665 22.95786263557803 17 22.562725019640855 27.742809035693018 26.867842860394013 22.826623084272118 18 22.4662527935563 27.22068288191246 27.990598478654455 22.322465845876785 19 23.723792501557504 26.686986798357086 27.447582096640566 22.14163860344484 20 23.938212940741867 26.384202597751376 27.523091236360266 22.154493225146492 21 23.815337441143985 26.122144393419756 27.160219147823057 22.902299017613206 22 23.934441000242693 26.303979142960532 27.11394785449483 22.647632002301947 23 23.407576917805862 26.408702219895623 27.179598823919072 23.004122038379442 24 23.354571914035773 26.309352396708242 27.441962056551862 22.89411363270412 25 23.461812396357086 26.35962133924626 27.307392214641663 22.87117404975499 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 922.0 1 922.0 2 1453.5 3 1985.0 4 1985.0 5 1985.0 6 5351.5 7 8718.0 8 8718.0 9 8718.0 10 11754.0 11 14790.0 12 14790.0 13 14790.0 14 23090.0 15 31390.0 16 31390.0 17 31390.0 18 54604.0 19 77818.0 20 77818.0 21 77818.0 22 116080.5 23 154343.0 24 154343.0 25 154343.0 26 216833.5 27 279324.0 28 279324.0 29 279324.0 30 355386.0 31 431448.0 32 431448.0 33 431448.0 34 501589.0 35 571730.0 36 571730.0 37 571730.0 38 640971.0 39 710212.0 40 710212.0 41 710212.0 42 768936.5 43 827661.0 44 827661.0 45 827661.0 46 863834.5 47 900008.0 48 900008.0 49 900008.0 50 893799.0 51 887590.0 52 887590.0 53 887590.0 54 814569.5 55 741549.0 56 741549.0 57 741549.0 58 644117.0 59 546685.0 60 546685.0 61 546685.0 62 451189.0 63 355693.0 64 355693.0 65 355693.0 66 275595.0 67 195497.0 68 195497.0 69 195497.0 70 143043.0 71 90589.0 72 90589.0 73 90589.0 74 62968.0 75 35347.0 76 35347.0 77 35347.0 78 23903.5 79 12460.0 80 12460.0 81 12460.0 82 8182.0 83 3904.0 84 3904.0 85 3904.0 86 2494.5 87 1085.0 88 1085.0 89 1085.0 90 701.0 91 317.0 92 317.0 93 317.0 94 206.0 95 95.0 96 95.0 97 95.0 98 67.5 99 40.0 100 40.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 15.409274545137478 3 0.0 4 0.0 5 5.086322153112829E-4 6 0.002223449398360751 7 0.0 8 0.0 9 0.0 10 0.0 11 4.3597047026681394E-5 12 0.0 13 0.0012788467127826542 14 0.0 15 0.0 16 2.1798523513340696E-4 17 0.0019473347671917689 18 0.0027030169156542464 19 1.5985583909783178E-4 20 2.615822821600884E-4 21 0.0 22 0.0010172644306225657 23 0.005536824972388537 24 1.7438818810672557E-4 25 1.4532349008893797E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6881200.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.333643273932296 #Duplication Level Percentage of deduplicated Percentage of total 1 76.17497133166957 48.24438460721979 2 15.024049489057429 19.030555817397357 3 4.2759477769980885 8.124340534990822 4 1.7015634425503874 4.310648483138017 5 0.8691821206102534 2.752423518340489 6 0.49454477633554556 1.8792793468453244 7 0.3114673593817669 1.3808453841390944 8 0.22332885890346782 1.131538422605327 9 0.1652618565236898 0.9419971921073235 >10 0.7145561828037669 7.730756447388161 >50 0.031268461509323824 1.3456288492678148 >100 0.012540481679368831 1.3998773777850702 >500 6.589626015305733E-4 0.2866656038410328 >1k 5.647708943913375E-4 0.7517749193484696 >5k 2.353212037771342E-5 0.07697786448791094 >10k+ 7.059636113314028E-5 0.6123056310980316 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 18171 0.26406731384060916 No Hit GGTATCAACGCAGAGTACTTTTTTT 11545 0.16777596930767888 No Hit TATCAACGCAGAGTACTTTTTTTTT 11368 0.16520374353310469 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.4532349008893797E-5 0.0 0.0 0.0 4 0.0 1.4532349008893797E-5 0.0 0.0 0.0 5 1.4532349008893797E-5 2.9064698017787595E-5 0.0 0.0 0.0 6 1.4532349008893797E-5 2.9064698017787595E-5 0.0 0.0 0.0 7 1.4532349008893797E-5 2.9064698017787595E-5 0.0 0.0 0.0 8 1.4532349008893797E-5 2.9064698017787595E-5 0.0 0.0 0.0 9 1.4532349008893797E-5 2.9064698017787595E-5 0.0 2.9064698017787595E-5 1.4532349008893797E-5 10 2.9064698017787595E-5 2.9064698017787595E-5 0.0 2.9064698017787595E-5 1.4532349008893797E-5 11 4.3597047026681394E-5 2.9064698017787595E-5 0.0 2.9064698017787595E-5 1.4532349008893797E-5 12 4.3597047026681394E-5 2.9064698017787595E-5 0.0 2.9064698017787595E-5 2.9064698017787595E-5 13 4.3597047026681394E-5 4.3597047026681394E-5 0.0 2.9064698017787595E-5 7.266174504446899E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3555 0.0 15.383527 1 GTATCAA 7500 0.0 11.858367 1 TATACCG 140 8.949428E-10 11.348488 5 CGCTTAA 80 0.0012902598 11.048169 1 CCGATCG 60 0.0066411425 10.903212 9 GTCCTAC 1940 0.0 10.763422 1 CGATCGC 70 0.0017109701 10.6806965 10 TAGGACC 1210 0.0 10.272671 4 TCCTACA 2470 0.0 9.929933 2 TGTAGGA 2550 0.0 9.488427 2 TCTATAC 570 0.0 9.345814 3 TTAGGAC 1565 0.0 9.315954 3 GTCCTAA 1260 0.0 9.294491 1 GTATTAG 560 0.0 9.272571 1 GTCAACG 310 0.0 9.266206 2 GTAGGAC 2460 0.0 9.193849 3 CGTGCGC 340 0.0 9.070739 10 TATACTG 1080 0.0 8.826601 5 GTCCTAG 845 0.0 8.760086 1 GTATTAA 770 0.0 8.752446 1 >>END_MODULE