FastQCFastQC Report
Thu 26 May 2016
SRR1513214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6881200
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT261640.3802243794686973No Hit
TATCAACGCAGAGTACTTTTTTTTT165610.24067023193629017No Hit
GGTATCAACGCAGAGTACTTTTTTT162480.23612160669650642No Hit
ACGCAGAGTACTTTTTTTTTTTTTT77120.11207347555658898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.013.9650491
TTAGGAC14650.011.2197063
TAGGACG20550.010.9112164
AGGACGT20150.010.7977525
GTATCAA97700.010.6436941
GGACGTG20550.010.2591686
TATACCG1303.8421786E-710.2319335
GTAGGAC22800.010.126153
GACGTGA12300.010.114337
TAGGACC9700.010.0887754
GTCCTAA14600.09.8016421
GTGGTAT13650.09.7848861
ACGTGAA14900.09.7516068
GTCCTAC22500.09.6250681
GGCGAGG11200.09.41316919
CTTACAC8950.09.3418443
GTATAGG8300.09.3103791
CGTGAAA14900.09.2415839
TATGCCG1651.06581865E-79.2131675
ATACCGC950.0018309598.9968386
Produced by FastQC (version 0.11.5)