FastQCFastQC Report
Thu 26 May 2016
SRR1513213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6281900
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT204770.3259682580111113No Hit
GGTATCAACGCAGAGTACTTTTTTT135620.21589009694519173No Hit
TATCAACGCAGAGTACTTTTTTTTT116470.1854056893614989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44100.015.3698781
CCGCGCA604.6974947E-412.4621389
GTATCAA87700.011.1903291
TCTTACG902.796858E-411.0392772
GTCAACG2500.011.0392772
ATACCGA1107.3494793E-610.1963776
GTGGTAT13150.09.9059671
TAGGACC10400.09.885914
ACCGCGC951.9391504E-49.8385318
GACCGCG1151.2468825E-59.7529797
GGACCGA1952.237357E-109.5863376
CTACGAC1805.5442797E-99.3466793
GTATTAA7450.09.3352271
GTATTAG4950.09.1436431
TGGTATC13350.09.0960342
TGGACCG3400.09.0717765
GTCCTAT5750.09.0234091
GTAGGAC20000.08.9260783
TAACCCG1055.2895804E-48.9015995
CTTATAC6550.08.8472383
Produced by FastQC (version 0.11.5)