FastQCFastQC Report
Thu 26 May 2016
SRR1513213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6281900
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT291940.4647320078320254No Hit
GGTATCAACGCAGAGTACTTTTTTT192070.3057514446266257No Hit
TATCAACGCAGAGTACTTTTTTTTT172570.2747098807685573No Hit
ACGCAGAGTACTTTTTTTTTTTTTT80380.12795491809802767No Hit
GTACTTTTTTTTTTTTTTTTTTTTT67640.1076744297107563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49650.014.2189141
GTATCAA108750.010.4393081
GTGGTAT14800.010.3781071
TGGTATC13900.010.3211472
GTAGGAC18900.09.9030793
TTAGGAC14400.09.698863
GTCCTAC17950.09.4604011
GTCCTAA11600.09.4578811
TAGGACC8450.09.4447054
TAGGACG18150.09.4224044
AGGACGT17450.09.4192545
GTATACG1054.3107805E-49.0858311
GGACGTG17900.08.9130946
TAGGACA10550.08.8255024
TGTAGGA23500.08.7327632
GTATTAG5050.08.6900131
GCGTATA1106.856329E-48.6728381
ATAAGAC5100.08.5694613
GATAGCG1000.00291613198.547027
GTGTTAA7150.08.5394111
Produced by FastQC (version 0.11.5)