FastQCFastQC Report
Thu 26 May 2016
SRR1513212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4644804
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT196280.42257972564611984No Hit
GGTATCAACGCAGAGTACTTTTTTT117750.2535090824069218No Hit
TATCAACGCAGAGTACTTTTTTTTT116890.2516575511044169No Hit
ACGCAGAGTACTTTTTTTTTTTTTT52460.11294340945279929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27250.015.2397251
GTCAACG1800.012.8855382
GTATCAA58250.011.888511
GGACCGT2150.010.432856
GTCCTAC9350.09.6863021
TAGGACC5350.09.6081344
GGTCTAC4450.09.4314691
GTATAAG4350.09.3943821
ATTATAC5300.09.3460953
GTAGGAC9250.09.1945373
TTAGTAC2550.09.1628383
GTGGTAT9200.09.1239221
GGGTATA2353.2196112E-98.9297961
TACGACT1055.292223E-48.9010434
GTCTTAC3650.08.7752791
TGGACCG2151.4188117E-98.6940425
GTATTAC4250.08.5759221
GTGTACG1951.330076E-68.4959591
GTATTAG3650.08.4726831
TGGTATC9000.08.4676392
Produced by FastQC (version 0.11.5)