FastQCFastQC Report
Thu 26 May 2016
SRR1513212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4644804
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT286880.6176363954216367No Hit
TATCAACGCAGAGTACTTTTTTTTT174720.3761622664809968No Hit
GGTATCAACGCAGAGTACTTTTTTT168050.36180213416970874No Hit
ACGCAGAGTACTTTTTTTTTTTTTT80320.17292441188045826No Hit
GTACTTTTTTTTTTTTTTTTTTTTT63470.13664731601161212No Hit
GAGTACTTTTTTTTTTTTTTTTTTT52630.11330940982655027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35200.014.0942911
GTATCAA82450.010.9466961
GTGGTAT9150.010.7398621
TAGGACC5100.010.0598674
GTATACG1106.529485E-59.54075051
CGTATAC800.004514449.5009853
ATACCGT1701.677472E-89.4965886
GTAGGAC9700.09.3050883
GTCCTAC9300.09.1303961
GTATAGG5250.09.086431
CGTGCGG950.0018312148.99657310
ATACGAC850.0074330738.9421043
TTTTACG1606.7394467E-78.9071732
TACACCG2252.6193447E-108.8675865
TATACCG1401.0847985E-58.8223445
AATACCG1201.7631934E-48.7092375
TGGTATC10450.08.63725952
GACCGTC1554.2321353E-68.5775637
TAAACCG1000.00290392438.5508875
GTGTAGA8350.08.45527651
Produced by FastQC (version 0.11.5)