FastQCFastQC Report
Thu 26 May 2016
SRR1513211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583912
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT43170.1670722532346303No Hit
GTCCTAAAGTGTGTATTTCTCATTT40450.15654557895160515No Hit
CTGTAGGACGTGGAATATGGCAAGA36030.13943973324168935No Hit
CTTTAGGACGTGAAATATGGCGAGG32710.12659099845505573No Hit
GTCCTACAGTGTGCATTTCTCATTT28690.11103319308087892No Hit
GTACATGGGAAGCAGTGGTATCAAC27120.10495713476310338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11450.012.5672634
GTAGGAC25950.011.3844073
GGACCGC752.1031711E-411.3833576
TGTAGGA26900.011.3443472
AGGACCT19850.010.9240335
GGACCTG18250.010.9155476
CGTAGGC700.001453434510.8912573
AGAACCG700.001478143710.8692765
TGGCGAG24200.010.86229718
AGACCGA700.001510275510.8412916
GTCCTAC23550.010.8293951
CTGTAGG27150.010.5896231
GGCGAGA15150.010.58619119
TGTCGAG1355.7825673E-810.54422718
GGACGTG22150.010.4925596
GGCGAGG10150.010.47171119
AGGACCG1659.131327E-1010.3752175
TCCTACA26300.010.3340592
AGGACGT22950.010.3187075
TAGGACT5500.010.2225334
Produced by FastQC (version 0.11.5)