##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513211_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2583912 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.72869199879872 31.0 30.0 34.0 25.0 34.0 2 29.558640154927875 31.0 30.0 34.0 23.0 34.0 3 29.76276978472951 31.0 30.0 34.0 25.0 34.0 4 32.85946657626111 37.0 35.0 37.0 25.0 37.0 5 33.0160678846648 37.0 35.0 37.0 26.0 37.0 6 33.36792313360517 37.0 35.0 37.0 28.0 37.0 7 33.321325958469174 37.0 35.0 37.0 27.0 37.0 8 33.21243138311212 37.0 35.0 37.0 27.0 37.0 9 34.484193347141854 39.0 35.0 39.0 26.0 39.0 10 34.07713149673828 39.0 34.0 39.0 23.0 39.0 11 34.5393531203849 39.0 35.0 39.0 27.0 39.0 12 34.533460891857 39.0 35.0 39.0 27.0 39.0 13 34.58299315146955 39.0 35.0 39.0 27.0 39.0 14 34.8310406855961 40.0 34.0 41.0 18.0 41.0 15 35.281225908622275 40.0 35.0 41.0 25.0 41.0 16 35.13363651703309 40.0 35.0 41.0 24.0 41.0 17 34.75406360588131 39.0 34.0 41.0 18.0 41.0 18 34.50461586927109 39.0 34.0 41.0 17.0 41.0 19 35.131318713640404 40.0 35.0 41.0 25.0 41.0 20 35.34736786701714 40.0 36.0 41.0 25.0 41.0 21 35.38417059094892 40.0 36.0 41.0 26.0 41.0 22 35.08361855976519 40.0 36.0 41.0 23.0 41.0 23 34.62836660072015 40.0 34.0 41.0 18.0 41.0 24 34.882124468635155 40.0 35.0 41.0 22.0 41.0 25 34.53287263652942 40.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8569345436961022 1101 2 1.1199213639899526 1101 3 1.0720718296879035 1101 4 1.3924890844837705 1101 5 1.25605732356388 1101 6 1.0371561431753165 1101 7 1.0351218406064646 1101 8 1.1110852434414369 1101 9 1.4304870610356204 1101 10 1.7485130214372404 1101 11 1.368940971442285 1101 12 1.2835603722071625 1101 13 1.1273423297250815 1101 14 2.106355369245655 1101 15 1.7589651778224962 1101 16 1.83775708689587 1101 17 2.247915162850518 1101 18 2.1898994706419614 1101 19 1.7628785655199337 1101 20 1.3211274196502742 1101 21 1.3788588626567702 1101 22 1.722627831661896 1101 23 1.9505354303753393 1101 24 1.6070724025795755 1101 25 1.9164647363217142 1102 1 0.9338645755225627 1102 2 1.0863813608779083 1102 3 1.013286856054581 1102 4 1.2649529241272148 1102 5 1.1976087109932791 1102 6 0.9600620093753136 1102 7 0.8963504530262583 1102 8 0.8983097608520794 1102 9 1.3304980285860637 1102 10 1.428166638775295 1102 11 1.1085707069392399 1102 12 0.9521219574020705 1102 13 0.9304063205836286 1102 14 1.9719753203166732 1102 15 1.5774042349147663 1102 16 1.6488971706194064 1102 17 2.0152453663191423 1102 18 2.011030718632675 1102 19 1.5056969792145694 1102 20 1.2899652500220284 1102 21 1.2461323090518377 1102 22 1.3728688093093382 1102 23 1.7570713364218236 1102 24 1.5295054988081773 1102 25 1.7614790592571197 1103 1 0.7327862080982541 1103 2 1.0519978064594042 1103 3 0.8601218209886454 1103 4 1.3342439792565628 1103 5 1.0596550264174383 1103 6 0.8357394160877121 1103 7 0.6639744791904292 1103 8 0.7704385058673324 1103 9 1.068616293462945 1103 10 1.3429006781000936 1103 11 0.9270207568650335 1103 12 0.8776550296424617 1103 13 0.8491354335468699 1103 14 1.698467272203878 1103 15 1.3222304673657135 1103 16 1.3875352726701067 1103 17 1.7059631976638059 1103 18 1.8912213520923657 1103 19 1.4640428447980227 1103 20 1.0838381905537204 1103 21 1.1444933298727378 1103 22 1.3258719451056677 1103 23 1.7128467799658011 1103 24 1.454563732090655 1103 25 1.7041126660668908 1104 1 0.6289122874466209 1104 2 0.869931551993929 1104 3 0.7649376042485585 1104 4 1.1165172396884273 1104 5 0.9541387509334953 1104 6 0.7801475025374813 1104 7 0.6254359740915092 1104 8 0.712960844886716 1104 9 1.1052474411994737 1104 10 1.1742666815533198 1104 11 0.8852365324986877 1104 12 0.801701273310897 1104 13 0.8826948649241331 1104 14 1.8161287920720284 1104 15 1.3382994674160074 1104 16 1.4145677776425387 1104 17 1.8217380449057075 1104 18 1.8629765101088793 1104 19 1.260989447316895 1104 20 1.0628139075326004 1104 21 1.0525845047531988 1104 22 1.337942116453931 1104 23 1.5855262813460271 1104 24 1.337032598754135 1104 25 1.5948641504610492 1105 1 0.5618804182675383 1105 2 0.7908005886192484 1105 3 0.7274081680412934 1105 4 1.0330263734377567 1105 5 0.9844667669877651 1105 6 0.6719554353501209 1105 7 0.6456702972292447 1105 8 0.6303579692427945 1105 9 0.8695745761753599 1105 10 1.2809720935496287 1105 11 0.7513916812906061 1105 12 0.7066364445326556 1105 13 0.6310128241506874 1105 14 1.5143666879747002 1105 15 1.137905220389115 1105 16 1.2679667099735568 1105 17 1.5411185304151829 1105 18 1.2297402639241 1105 19 1.0404741718245418 1105 20 0.8243427101850003 1105 21 0.7818282756528063 1105 22 0.9615708954335958 1105 23 1.27843518031667 1105 24 1.092896206978132 1105 25 1.3276307866085943 1106 1 0.6681628554596237 1106 2 0.8546408229899605 1106 3 0.732470107305339 1106 4 1.2496766424447188 1106 5 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1107 18 1.8841808672855649 1107 19 1.3681517700057384 1107 20 1.174623005429325 1107 21 1.0341420090816484 1107 22 1.2564383780708681 1107 23 1.5419615021163722 1107 24 1.2974824081470615 1107 25 1.67617999918658 1108 1 0.6836687019263223 1108 2 0.8569687067563159 1108 3 0.7524203387558508 1108 4 1.1705464325784192 1108 5 1.0302566012857426 1108 6 0.7212107679261663 1108 7 0.725426342017478 1108 8 0.7191960716931618 1108 9 0.9798151055563693 1108 10 1.1822666969646392 1108 11 0.8270547598503128 1108 12 0.6143085023751027 1108 13 0.6265213797122584 1108 14 1.6896502205471364 1108 15 1.1428402197580425 1108 16 1.3050828816978353 1108 17 1.6046929340501705 1108 18 1.8336150588250177 1108 19 1.3069533193336014 1108 20 1.1115562476257281 1108 21 1.087105858098127 1108 22 1.2803390388136293 1108 23 1.6596724461431052 1108 24 1.3670293816414087 1108 25 1.6178599566560123 1109 1 0.4213894081863572 1109 2 0.5314620562348829 1109 3 0.5796597856877881 1109 4 0.7838089897203346 1109 5 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2216 25 -1.4763459372256094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 103759.0 3 4.0 4 928.0 5 5270.0 6 6433.0 7 5734.0 8 5351.0 9 5103.0 10 4734.0 11 4539.0 12 4476.0 13 4418.0 14 4338.0 15 4561.0 16 4722.0 17 5019.0 18 5725.0 19 7031.0 20 9115.0 21 11602.0 22 14455.0 23 17253.0 24 19898.0 25 22879.0 26 25747.0 27 29102.0 28 32708.0 29 39257.0 30 47241.0 31 56535.0 32 72266.0 33 94200.0 34 125334.0 35 169661.0 36 243717.0 37 359607.0 38 1011190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83179548966384 19.013361058712523 12.606441498752124 25.548401952871508 2 13.891283716303096 20.399299425681157 42.300666507844994 23.40875035017076 3 18.072567778143373 25.2052201742024 30.183778565530773 26.53843348212346 4 11.036935059906092 17.14002011578825 39.917359645333846 31.905685178971808 5 12.232374373433705 38.43057631877194 36.83614236532923 12.500906942465123 6 31.150604641188686 37.864926243490736 18.63884219176576 12.34562692355482 7 27.40140735215506 31.766297016411883 23.060439345630407 17.771856285802652 8 24.362674437148435 37.45647296717725 21.227915208023994 16.952937387650323 9 26.315400354721664 15.081128451510665 20.604706758523733 37.99876443524394 10 13.45043342543511 29.12168350382097 36.36767266373355 21.060210407010366 11 33.79733551960542 22.471300340288913 25.486095224381266 18.245268915724395 12 22.97998196014974 24.841764188961623 32.47362736482693 19.7046264860617 13 28.628080694213033 22.268564686061854 26.106618808416282 22.996735811308834 14 19.863985711067187 22.21868663524898 29.136927089744812 28.780400563939025 15 22.58390241371647 31.37908845441149 23.61297061895864 22.424038512913395 16 21.470869445724 28.206064171992395 28.727089034865312 21.595977347418295 17 20.998938362290257 28.697012004791695 28.90755853526519 21.39649109765286 18 20.864210626563544 27.610939523993505 30.745692922463704 20.77915692697925 19 23.0945072676011 27.19147778104249 27.95797577943749 21.75603917191892 20 23.356050153535975 27.649947272820185 28.21976471754181 20.77423785610203 21 22.70647414110497 27.106204841736293 27.383208433297884 22.804112583860853 22 22.687156955061887 28.408147588308903 27.485759376045397 21.418936080583812 23 21.592406126663917 28.072013270696267 28.39592200949621 21.9396585931436 24 22.715874805622843 27.400248121278953 28.43187971229083 21.451997360807372 25 22.269730843699172 27.5372901166624 28.304018354399258 21.888960685239166 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 223.0 1 223.0 2 355.0 3 487.0 4 487.0 5 487.0 6 1066.5 7 1646.0 8 1646.0 9 1646.0 10 2721.5 11 3797.0 12 3797.0 13 3797.0 14 7484.0 15 11171.0 16 11171.0 17 11171.0 18 21363.5 19 31556.0 20 31556.0 21 31556.0 22 51248.0 23 70940.0 24 70940.0 25 70940.0 26 102138.0 27 133336.0 28 133336.0 29 133336.0 30 172102.0 31 210868.0 32 210868.0 33 210868.0 34 240048.0 35 269228.0 36 269228.0 37 269228.0 38 289947.5 39 310667.0 40 310667.0 41 310667.0 42 321892.5 43 333118.0 44 333118.0 45 333118.0 46 337540.0 47 341962.0 48 341962.0 49 341962.0 50 322364.5 51 302767.0 52 302767.0 53 302767.0 54 264371.0 55 225975.0 56 225975.0 57 225975.0 58 190744.5 59 155514.0 60 155514.0 61 155514.0 62 124775.5 63 94037.0 64 94037.0 65 94037.0 66 71840.5 67 49644.0 68 49644.0 69 49644.0 70 36161.5 71 22679.0 72 22679.0 73 22679.0 74 15766.5 75 8854.0 76 8854.0 77 8854.0 78 6180.5 79 3507.0 80 3507.0 81 3507.0 82 2428.5 83 1350.0 84 1350.0 85 1350.0 86 883.5 87 417.0 88 417.0 89 417.0 90 274.5 91 132.0 92 132.0 93 132.0 94 79.5 95 27.0 96 27.0 97 27.0 98 18.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16742056231017155 2 0.2553492533801461 3 0.25852273606841103 4 0.4567880020681819 5 0.2543043261535223 6 0.01853778302047438 7 0.01853778302047438 8 0.01780246386099836 9 0.01849908201208091 10 0.020821142515689388 11 0.020085823356213367 12 0.02871614822795823 13 0.028174334110449582 14 0.025310459489332453 15 0.04218409914888742 16 0.020975946549263286 17 0.042958119316756915 18 0.0324314450337318 19 0.028445241169203904 20 0.03111561074835366 21 0.0353340206632424 22 0.04489316973643065 23 0.03007068352172984 24 0.05046611494509101 25 0.038275297301146476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2583912.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.08728046572173 #Duplication Level Percentage of deduplicated Percentage of total 1 84.39229536330599 62.52395655727278 2 11.027935494511418 16.3405949987951 3 2.5620601858721366 5.694482146823043 4 0.8568832474287685 2.539365979145345 5 0.37750313589238654 1.3984090352774352 6 0.20744528964597636 0.9221434413176598 7 0.11835430753926435 0.6137984143891434 8 0.07885741944164697 0.46738654007810854 9 0.061001148553415034 0.4067468281447214 >10 0.28230721521768043 3.737648902043573 >50 0.020293779921830495 1.0239181064550014 >100 0.012110462703476646 1.7625714905658865 >500 0.0020565187263990423 1.0432100699174163 >1k 8.964312396787608E-4 1.5257674897748594 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4317 0.1670722532346303 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4045 0.15654557895160515 No Hit CTGTAGGACGTGGAATATGGCAAGA 3603 0.13943973324168935 No Hit CTTTAGGACGTGAAATATGGCGAGG 3271 0.12659099845505573 No Hit GTCCTACAGTGTGCATTTCTCATTT 2869 0.11103319308087892 No Hit GTACATGGGAAGCAGTGGTATCAAC 2712 0.10495713476310338 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.87010083934747E-5 0.0 4 0.0 0.0 0.0 3.87010083934747E-5 0.0 5 0.0 0.0 0.0 3.87010083934747E-5 0.0 6 0.0 0.0 0.0 7.74020167869494E-5 0.0 7 0.0 0.0 0.0 7.74020167869494E-5 0.0 8 0.0 0.0 0.0 7.74020167869494E-5 0.0 9 0.0 0.0 0.0 1.548040335738988E-4 0.0 10 3.87010083934747E-5 0.0 0.0 1.548040335738988E-4 0.0 11 3.87010083934747E-5 0.0 0.0 3.096080671477976E-4 0.0 12 7.74020167869494E-5 0.0 0.0 3.483090755412723E-4 0.0 13 7.74020167869494E-5 0.0 0.0 3.483090755412723E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1145 0.0 12.567263 4 GTAGGAC 2595 0.0 11.384407 3 GGACCGC 75 2.1031711E-4 11.383357 6 TGTAGGA 2690 0.0 11.344347 2 AGGACCT 1985 0.0 10.924033 5 GGACCTG 1825 0.0 10.915547 6 CGTAGGC 70 0.0014534345 10.891257 3 AGAACCG 70 0.0014781437 10.869276 5 TGGCGAG 2420 0.0 10.862297 18 AGACCGA 70 0.0015102755 10.841291 6 GTCCTAC 2355 0.0 10.829395 1 CTGTAGG 2715 0.0 10.589623 1 GGCGAGA 1515 0.0 10.586191 19 TGTCGAG 135 5.7825673E-8 10.544227 18 GGACGTG 2215 0.0 10.492559 6 GGCGAGG 1015 0.0 10.471711 19 AGGACCG 165 9.131327E-10 10.375217 5 TCCTACA 2630 0.0 10.334059 2 AGGACGT 2295 0.0 10.318707 5 TAGGACT 550 0.0 10.222533 4 >>END_MODULE