FastQCFastQC Report
Thu 26 May 2016
SRR1513211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583912
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT44380.17175507525024072No Hit
GTCCTAAAGTGTGTATTTCTCATTT41090.15902244348878755No Hit
CTGTAGGACGTGGAATATGGCAAGA39690.1536043023137011No Hit
CTTTAGGACGTGAAATATGGCGAGG39500.15286898315422506No Hit
GTATCAACGCAGAGTACTTTTTTTT35470.13727247677165474No Hit
GTACATGGGAAGCAGTGGTATCAAC30930.11970221896101725No Hit
GTCCTACAGTGTGCATTTCTCATTT28810.11149760518160061No Hit
CCCATGTACTCTGCGTTGATACCAC28220.10921424568638562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8450.012.393584
TCCAACG1501.4551915E-1112.021749518
ACGAAAT1850.011.28642112
GGCGAGG10050.011.23531719
CCAACGA1303.3272954E-810.94844319
TTAGGAC14100.010.7365343
GTCCTAC26600.010.4193551
GGACGTG22600.010.3197186
TGTAGGA25700.010.2998652
AGGACGT22650.010.2981385
CGAAATC1951.6370905E-1110.22092113
CTGTAGG25500.010.196521
AAGACCG750.002601587710.1552255
AGGACCT15950.010.1472665
GTAGGAC24650.010.08114053
GGACCTG15600.010.0075886
CCTACAC3450.09.9350383
TGGCGAG23900.09.92767118
TCTATAC2600.09.8872733
GTATTAA4350.09.8526431
Produced by FastQC (version 0.11.5)