##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513211_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2583912 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.899172649842566 31.0 31.0 34.0 28.0 34.0 2 31.010688057488025 33.0 31.0 34.0 27.0 34.0 3 31.11374381170876 33.0 31.0 34.0 27.0 34.0 4 34.50024381635288 37.0 35.0 37.0 31.0 37.0 5 34.263684676567934 37.0 35.0 37.0 30.0 37.0 6 34.185148720235055 37.0 35.0 37.0 30.0 37.0 7 34.05362914836109 37.0 35.0 37.0 30.0 37.0 8 34.02379531501073 37.0 35.0 37.0 30.0 37.0 9 35.60036835619789 39.0 37.0 39.0 30.0 39.0 10 35.447850004179706 39.0 35.0 39.0 30.0 39.0 11 35.45697221886814 39.0 37.0 39.0 30.0 39.0 12 35.40153108929406 39.0 35.0 39.0 30.0 39.0 13 35.371502976881565 39.0 35.0 39.0 30.0 39.0 14 36.54063992891399 40.0 37.0 41.0 29.0 41.0 15 36.52667389601503 40.0 37.0 41.0 29.0 41.0 16 36.501626603382775 40.0 37.0 41.0 29.0 41.0 17 36.43755243986637 40.0 37.0 41.0 29.0 41.0 18 36.40022028613978 40.0 37.0 41.0 29.0 41.0 19 36.480666137236874 40.0 37.0 41.0 29.0 41.0 20 36.44571486954664 40.0 37.0 41.0 29.0 41.0 21 36.40082092579004 40.0 37.0 41.0 29.0 41.0 22 36.341001551136415 40.0 37.0 41.0 29.0 41.0 23 36.262188108573355 40.0 37.0 41.0 29.0 41.0 24 36.20464474022335 40.0 37.0 41.0 29.0 41.0 25 36.15120793587398 40.0 37.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48694644012714505 1101 2 0.5120040314028991 1101 3 0.40300444671629876 1101 4 0.614688088490631 1101 5 0.466742891478404 1101 6 0.4214310467167408 1101 7 0.475772186506795 1101 8 0.5283574568587852 1101 9 0.6428260692329886 1101 10 0.6869318188733757 1101 11 0.5561652482954003 1101 12 0.5723140990253412 1101 13 0.5866881118418874 1101 14 0.692027750181154 1101 15 0.6432913979974799 1101 16 0.6026428350268063 1101 17 0.6473585802261468 1101 18 0.6222945851347106 1101 19 0.6748206138670483 1101 20 0.6791669944556205 1101 21 0.669899557836068 1101 22 0.7267064670179835 1101 23 0.8122550976917253 1101 24 0.7488709270195741 1101 25 0.7321486233160073 1102 1 0.3820952183762998 1102 2 0.41454692652089875 1102 3 0.41889546765129637 1102 4 0.48476813104472427 1102 5 0.48628123986127036 1102 6 0.48674870467859677 1102 7 0.4918774859586179 1102 8 0.52528962735758 1102 9 0.5735869636093085 1102 10 0.5563553849861123 1102 11 0.5661904805161413 1102 12 0.5759899421043713 1102 13 0.512643642537661 1102 14 0.6166209740172093 1102 15 0.6102356122376094 1102 16 0.6250444680950693 1102 17 0.7061897546657221 1102 18 0.6479480283629044 1102 19 0.6679542418162043 1102 20 0.640880715341055 1102 21 0.6050089488696813 1102 22 0.5900124705865508 1102 23 0.6052452563716173 1102 24 0.7344371508028402 1102 25 0.7185927309652627 1103 1 0.37439540389963355 1103 2 0.31601774170871266 1103 3 0.3929957609952055 1103 4 0.49457275727896644 1103 5 0.40947433751218654 1103 6 0.2682304972754892 1103 7 0.33998762488522516 1103 8 0.42185853360386716 1103 9 0.535515643727436 1103 10 0.5681548198543283 1103 11 0.5138224595087095 1103 12 0.4852013261410377 1103 13 0.4156037100565442 1103 14 0.6222087015756443 1103 15 0.5226849816664227 1103 16 0.6091316035025827 1103 17 0.6586766908685533 1103 18 0.6110163489250695 1103 19 0.47502533421458537 1103 20 0.4593676799709101 1103 21 0.3768456298659473 1103 22 0.4556356726366886 1103 23 0.48963823818580465 1103 24 0.5759498641797691 1103 25 0.5829058651967571 1104 1 0.2995035080801216 1104 2 0.40548431408470265 1104 3 0.4133304426693414 1104 4 0.5216677790577435 1104 5 0.41717976519355204 1104 6 0.35885330014182415 1104 7 0.4579458021991414 1104 8 0.482340966414327 1104 9 0.4524483868122431 1104 10 0.6086401353107647 1104 11 0.6125315403259606 1104 12 0.5583161850443759 1104 13 0.586782325660856 1104 14 0.577458299264137 1104 15 0.5060950963437918 1104 16 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-0.4222681706840419 2214 7 -0.48889023561655165 2214 8 -0.5692790454749712 2214 9 -0.6295147650100219 2214 10 -0.6264582561357201 2214 11 -0.5593929054575497 2214 12 -0.6747458436049811 2214 13 -0.6477634314688956 2214 14 -0.7217263803062295 2214 15 -0.6973444707947891 2214 16 -0.8111569178365983 2214 17 -0.7756712996595567 2214 18 -0.7782283174827569 2214 19 -0.7883443969105457 2214 20 -0.7578364466167073 2214 21 -0.8191822872288697 2214 22 -0.928796755177764 2214 23 -0.8823537042868423 2214 24 -0.9746857343090909 2214 25 -0.9401626579708733 2215 1 -0.43293081316659254 2215 2 -0.4321226357304866 2215 3 -0.46362021537407827 2215 4 -0.321007203926321 2215 5 -0.5134811820566227 2215 6 -0.5606883912079894 2215 7 -0.5884154732364877 2215 8 -0.5714193784771311 2215 9 -0.7507048575574515 2215 10 -0.7196441080828748 2215 11 -0.7756034759814057 2215 12 -0.7559903530912848 2215 13 -0.811577418490451 2215 14 -0.9438107508204467 2215 15 -1.0376159254144213 2215 16 -0.9236360630777014 2215 17 -0.9556786454084403 2215 18 -1.0021501131385548 2215 19 -0.9144114761553936 2215 20 -0.9404983936506994 2215 21 -0.8653846547070856 2215 22 -1.0332892097039874 2215 23 -0.9582332484947642 2215 24 -0.9301411548230263 2215 25 -0.9400203972035968 2216 1 -0.27683717459873947 2216 2 -0.22376169930256395 2216 3 -0.25866379262108197 2216 4 -0.11596370799689737 2216 5 -0.34474720069609077 2216 6 -0.30231059458284193 2216 7 -0.6552861942287933 2216 8 -0.5272164613303474 2216 9 -0.6695706631659846 2216 10 -0.5121894402583322 2216 11 -0.4950755622430947 2216 12 -0.5165044787472226 2216 13 -0.5453790882889038 2216 14 -0.659964921351019 2216 15 -0.7147998595526772 2216 16 -0.8678366251828464 2216 17 -0.8389247101919395 2216 18 -0.7314097207843915 2216 19 -0.845056344641101 2216 20 -0.7638298228853415 2216 21 -1.0576833094713933 2216 22 -0.8810120586876309 2216 23 -0.9710047554901351 2216 24 -0.8584502549890729 2216 25 -1.0440967630723037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54957.0 3 1.0 4 976.0 5 7984.0 6 9029.0 7 6830.0 8 5540.0 9 4896.0 10 4518.0 11 4067.0 12 3733.0 13 3630.0 14 3518.0 15 3424.0 16 3636.0 17 3774.0 18 4164.0 19 4980.0 20 6497.0 21 8333.0 22 10774.0 23 13204.0 24 15195.0 25 16908.0 26 18786.0 27 20850.0 28 23392.0 29 28046.0 30 35679.0 31 46000.0 32 59047.0 33 76881.0 34 102049.0 35 137805.0 36 200054.0 37 327318.0 38 1307437.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.24064079898266 19.760747185261003 13.637759374709221 27.360852641047114 2 13.396334350236216 20.8067076885444 40.69986708787759 25.09709087334179 3 16.640606434503542 25.24067656491049 30.298000044895844 27.820716955690123 4 10.923106574577126 17.596992409037323 38.53433348994644 32.945567526439106 5 12.19137464010652 38.59920976416085 36.634890438239296 12.574525157493335 6 31.191432032426892 38.28473699740647 18.198894255408366 12.324936714758273 7 27.11414571302263 31.83743528480878 23.107320181384477 17.941098820784116 8 24.435241022147565 37.701716223225134 20.8079873663676 17.055055388259703 9 26.12183605542225 14.846853471630931 20.285083984828546 38.746226488118275 10 13.964263819826739 29.489431168686416 35.53670838261429 21.009596628872554 11 33.80494382161622 22.33257169748815 25.36375077789027 18.498733703005364 12 22.762780916215767 24.94284826373664 32.45932232186016 19.835048498187437 13 28.615757812185556 22.145723228964453 26.028905009148918 23.209613949701076 14 20.349748728160055 22.300593493055917 28.07847189567526 29.271185883108764 15 22.60278213809139 31.444143608605867 23.40393171284471 22.549142540458035 16 21.64110078052194 28.380533083170018 28.20757827665958 21.770787859648472 17 21.214864785356625 29.010183289928808 28.219031310094106 21.55592061462046 18 21.171251383931683 27.861273913952356 30.010994030705863 20.956480671410098 19 23.016960329918355 27.296556539077184 27.842047252383207 21.844435878621255 20 23.221882169361805 27.65682422621204 28.25494831093319 20.866345293492966 21 22.542881248355187 27.057912906172117 27.474921435979997 22.9242844094927 22 22.64802874097249 28.431673430971006 27.34254754524834 21.577750282808168 23 21.73170272929305 28.13117078845986 27.971596279200817 22.165530203046274 24 22.60463978649428 27.348377189316043 28.469893711550547 21.57708931263913 25 22.290245852596804 27.595237597652545 28.014409192904075 22.100107356846575 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 123.0 1 123.0 2 272.0 3 421.0 4 421.0 5 421.0 6 1012.5 7 1604.0 8 1604.0 9 1604.0 10 2509.5 11 3415.0 12 3415.0 13 3415.0 14 6839.5 15 10264.0 16 10264.0 17 10264.0 18 20133.5 19 30003.0 20 30003.0 21 30003.0 22 49249.0 23 68495.0 24 68495.0 25 68495.0 26 99605.0 27 130715.0 28 130715.0 29 130715.0 30 168820.0 31 206925.0 32 206925.0 33 206925.0 34 236107.5 35 265290.0 36 265290.0 37 265290.0 38 286351.5 39 307413.0 40 307413.0 41 307413.0 42 319361.0 43 331309.0 44 331309.0 45 331309.0 46 338115.0 47 344921.0 48 344921.0 49 344921.0 50 326461.5 51 308002.0 52 308002.0 53 308002.0 54 269222.0 55 230442.0 56 230442.0 57 230442.0 58 194593.0 59 158744.0 60 158744.0 61 158744.0 62 128065.5 63 97387.0 64 97387.0 65 97387.0 66 74214.5 67 51042.0 68 51042.0 69 51042.0 70 37016.0 71 22990.0 72 22990.0 73 22990.0 74 16019.5 75 9049.0 76 9049.0 77 9049.0 78 6234.0 79 3419.0 80 3419.0 81 3419.0 82 2408.5 83 1398.0 84 1398.0 85 1398.0 86 915.5 87 433.0 88 433.0 89 433.0 90 265.5 91 98.0 92 98.0 93 98.0 94 53.0 95 8.0 96 8.0 97 8.0 98 5.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17926307087857482 2 0.009675252098368675 3 0.005959955292595104 4 0.0019350504196737348 5 0.07345451393081498 6 0.3201347414308227 7 0.025929675623628048 8 0.0128487347866336 9 0.004334512940069166 10 0.003405688738625774 11 0.0 12 3.8701008393474705E-5 13 0.0 14 0.0022059574784280577 15 0.0 16 0.0 17 0.017957267894572262 18 0.02678109780828449 19 0.0 20 0.0 21 0.0012384322685911906 22 1.935050419673735E-4 23 0.0064243673933168 24 0.0 25 2.322060503608482E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2583912.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.52259629875797 #Duplication Level Percentage of deduplicated Percentage of total 1 82.85459302441393 59.259756083830474 2 11.79663482327329 16.874519002976914 3 2.9617022569881977 6.354859046510616 4 1.0547438228136141 3.0175206655082736 5 0.4520035851487766 1.6164234973093612 6 0.222105023587089 0.9531316762767292 7 0.1364780754378718 0.683288640522204 8 0.09641893228312379 0.5516905895394516 9 0.06436759330099584 0.4143363651350788 >10 0.3213395843702458 4.121673771665018 >50 0.021849608685417965 1.082551510566015 >100 0.014359837579804809 2.0000240257968818 >500 0.0022692214118226564 1.182321278613519 >1k 0.0011346107058528526 1.8879038457494617 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4438 0.17175507525024072 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4109 0.15902244348878755 No Hit CTGTAGGACGTGGAATATGGCAAGA 3969 0.1536043023137011 No Hit CTTTAGGACGTGAAATATGGCGAGG 3950 0.15286898315422506 No Hit GTATCAACGCAGAGTACTTTTTTTT 3547 0.13727247677165474 No Hit GTACATGGGAAGCAGTGGTATCAAC 3093 0.11970221896101725 No Hit GTCCTACAGTGTGCATTTCTCATTT 2881 0.11149760518160061 No Hit CCCATGTACTCTGCGTTGATACCAC 2822 0.10921424568638562 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.87010083934747E-5 0.0 5 0.0 0.0 0.0 3.87010083934747E-5 0.0 6 0.0 0.0 0.0 3.87010083934747E-5 0.0 7 0.0 0.0 0.0 3.87010083934747E-5 0.0 8 0.0 0.0 0.0 3.87010083934747E-5 0.0 9 0.0 0.0 0.0 1.1610302518042411E-4 0.0 10 7.74020167869494E-5 0.0 0.0 1.935050419673735E-4 3.87010083934747E-5 11 7.74020167869494E-5 0.0 0.0 3.096080671477976E-4 3.87010083934747E-5 12 7.74020167869494E-5 0.0 0.0 3.096080671477976E-4 3.87010083934747E-5 13 7.74020167869494E-5 0.0 0.0 3.096080671477976E-4 3.87010083934747E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 845 0.0 12.39358 4 TCCAACG 150 1.4551915E-11 12.0217495 18 ACGAAAT 185 0.0 11.286421 12 GGCGAGG 1005 0.0 11.235317 19 CCAACGA 130 3.3272954E-8 10.948443 19 TTAGGAC 1410 0.0 10.736534 3 GTCCTAC 2660 0.0 10.419355 1 GGACGTG 2260 0.0 10.319718 6 TGTAGGA 2570 0.0 10.299865 2 AGGACGT 2265 0.0 10.298138 5 CGAAATC 195 1.6370905E-11 10.220921 13 CTGTAGG 2550 0.0 10.19652 1 AAGACCG 75 0.0026015877 10.155225 5 AGGACCT 1595 0.0 10.147266 5 GTAGGAC 2465 0.0 10.0811405 3 GGACCTG 1560 0.0 10.007588 6 CCTACAC 345 0.0 9.935038 3 TGGCGAG 2390 0.0 9.927671 18 TCTATAC 260 0.0 9.887273 3 GTATTAA 435 0.0 9.852643 1 >>END_MODULE