FastQCFastQC Report
Thu 26 May 2016
SRR1513210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219236
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC28900.13022499635009524No Hit
GTCCTACAGTGGACATTTCTAAATT26600.11986106930493197No Hit
CCCATGTACTCTGCGTTGATACCAC24560.11066871662139582No Hit
GTCCTAAAGTGTGTATTTCTCATTT23570.10620772193673858No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGT350.002180855516.27421812
TAAACGT400.005218956614.2750154
TAGGACC6500.011.5664224
GGCGAGG7950.011.22426919
GTCCTAA7950.011.0352041
TCCAACG1900.010.9917418
GGACGGG951.3745881E-510.99000556
TTAGGAC10450.010.8371733
GTCCTAC15950.010.58210851
TGGCGAG15350.010.57511418
TGTAGGA17600.010.5502442
AGGACGT15000.010.3976165
GGACGTG14900.010.3831746
CGCAGAT1659.440555E-1010.3544538
TCCTACA17150.010.3273622
TAGGACG16000.010.28990654
CTGTAGG17300.010.2524221
AGGACCT11250.010.228555
GTAGGAC16500.010.0934463
ACGAAAT1356.507671E-79.84489712
Produced by FastQC (version 0.11.5)