FastQCFastQC Report
Thu 26 May 2016
SRR1513210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219236
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC31820.14338267764221563No Hit
GTATCAACGCAGAGTACTTTTTTTT31380.1414000133379235No Hit
CCCATGTACTCTGCGTTGATACCAC31290.14099446836659105No Hit
GTCCTACAGTGGACATTTCTAAATT24960.11247113871620684No Hit
CTTTAGGACGTGAAATATGGCGAGG23830.10737929629836575No Hit
TATCAACGCAGAGTACTTTTTTTTT23630.10647808525096024No Hit
CTGTAGGACGTGGAATATGGCAAGA22840.1029183016137085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAA250.006067914418.98095711
TAGGACC6300.012.5406354
GGCGAGG6050.011.44899819
CTAATAC2450.011.2679083
GGACCGG1201.2436067E-711.1040056
GTCCTAA7700.010.7584071
GTACCGT803.7076417E-410.7074346
TACCGTA1002.4239285E-510.4404677
AGGACCT12650.010.384145
TCCAACG1201.540051E-610.28343818
GTAGGAC15550.010.2234513
TGGCGAG15750.010.12523118
GGACCTG12050.010.1101086
ATACCGT856.483274E-410.0775856
TTATACT2002.3646862E-119.9947344
TGTAGGA16150.09.9032482
GTATAGC2600.09.888031
AGGACGT13800.09.7257255
AATCCCG2350.09.69043819
GTCCTAC15350.09.6148551
Produced by FastQC (version 0.11.5)