FastQCFastQC Report
Thu 26 May 2016
SRR1513209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626401
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT32280.12290583197310692No Hit
GTACATGGGAAGCAGTGGTATCAAC27280.1038683734890445No Hit
CTGTAGGACGTGGAATATGGCAAGA26750.10185040288973389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6400.012.8032694
ACCGCTC600.0059317411.0696598
GCCGGTT951.37836705E-510.98747411
GTCCTAC16400.010.89133551
GTAGGAC15800.010.4928773
GTATTAA3100.010.4761241
GGACTGT4550.010.2173686
AGGACGT15850.010.2009855
CCGCCGG750.002671226910.122951519
CGTGCGC1502.4905603E-810.12179510
GGACGTG15850.010.116076
TTATACT4150.010.1019914
GTATTAC3150.010.0066061
TCCTACA19500.09.8275742
CCTACAC3700.09.7855293
CCCCTAC2159.094947E-129.7738941
GTCCTAA9200.09.7594071
TATACTG4100.09.7428925
TTAGGAC11700.09.69088653
TAGGACT4950.09.6242574
Produced by FastQC (version 0.11.5)